{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Henning Y"],"funding":["Bundesministerium für Bildung und Forschung (Federal Ministry of Education and Research)","EC | Horizon 2020 Framework Programme (EU Framework Programme for Research and Innovation H2020)"],"pagination":["662"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9338085"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(7)"],"pubmed_abstract":["Oxidative stress and hypoxia in the retinal pigment epithelium (RPE) have long been considered major risk factors in the pathophysiology of age-related macular degeneration (AMD), but systematic investigation of the interplay between these two risk factors was lacking. For this purpose, we treated a human RPE cell line (ARPE-19) with sodium iodate (SI), an oxidative stress agent, together with dimethyloxalylglycine (DMOG) which leads to stabilization of hypoxia-inducible factors (HIFs), key regulators of cellular adaptation to hypoxic conditions. We found that HIF stabilization aggravated oxidative stress-induced cell death by SI and iron-dependent ferroptosis was identified as the main cell death mechanism. Ferroptotic cell death depends on the Fenton reaction where H<sub>2</sub>O<sub>2</"],"journal":["Cell death & disease"],"pubmed_title":["Hypoxia aggravates ferroptosis in RPE cells by promoting the Fenton reaction."],"pmcid":["PMC9338085"],"funding_grant_id":["02NUK061B","860245"],"pubmed_authors":["Larafa S","Matschke J","Henning Y","Blind US","Fandrey J"],"additional_accession":[]},"is_claimable":false,"name":"Hypoxia aggravates ferroptosis in RPE cells by promoting the Fenton reaction.","description":"Oxidative stress and hypoxia in the retinal pigment epithelium (RPE) have long been considered major risk factors in the pathophysiology of age-related macular degeneration (AMD), but systematic investigation of the interplay between these two risk factors was lacking. For this purpose, we treated a human RPE cell line (ARPE-19) with sodium iodate (SI), an oxidative stress agent, together with dimethyloxalylglycine (DMOG) which leads to stabilization of hypoxia-inducible factors (HIFs), key regulators of cellular adaptation to hypoxic conditions. We found that HIF stabilization aggravated oxidative stress-induced cell death by SI and iron-dependent ferroptosis was identified as the main cell death mechanism. Ferroptotic cell death depends on the Fenton reaction where H<sub>2</sub>O<sub>2</","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jul","modification":"2025-04-27T03:25:01.638Z","creation":"2025-04-06T18:47:08.238Z"},"accession":"S-EPMC9338085","cross_references":{"pubmed":["35906211"],"doi":["10.1038/s41419-022-05121-z"]}}