biostudies-literatureAdderley JYourgene Health Singaporethe Australian Research Councilthe Australian National Health and Medical Research Council (NHMRC)the Australian National Health and Medical Research Council273https://www.ebi.ac.uk/biostudies/studies/S-EPMC9338626biostudies-literatureUnknown15(1)<h4>Background</h4>Gaining insight into molecular signalling pathways of socioeconomically important parasitic nematodes has implications for understanding their molecular biology and for developing novel anthelmintic interventions.<h4>Methods</h4>Here, we evaluated the use of a human antibody-based microarray to explore conserved elements of the signalome in the barber's pole worm Haemonchus contortus. To do this, we prepared extracts from mixed-sex (female and male) adult worms and third-stage larvae (L3s), incubated these extracts on the antibody microarray and then measured the amounts of antibody-bound proteins ('signal intensity').<h4>Results</h4>In total, 878 signals were classified into two distinct categories: signals that were higher for adults than for larvae of H. contortus (n Parasites & vectorsRepurposing of a human antibody-based microarray to explore conserved components of the signalome of the parasitic nematode Haemonchus contortus.PMC9338626APP2003712Zheng YGasser RBWang TAdderley JDoerig CYoung NDMa GfalseRepurposing of a human antibody-based microarray to explore conserved components of the signalome of the parasitic nematode Haemonchus contortus.<h4>Background</h4>Gaining insight into molecular signalling pathways of socioeconomically important parasitic nematodes has implications for understanding their molecular biology and for developing novel anthelmintic interventions.<h4>Methods</h4>Here, we evaluated the use of a human antibody-based microarray to explore conserved elements of the signalome in the barber's pole worm Haemonchus contortus. To do this, we prepared extracts from mixed-sex (female and male) adult worms and third-stage larvae (L3s), incubated these extracts on the antibody microarray and then measured the amounts of antibody-bound proteins ('signal intensity').<h4>Results</h4>In total, 878 signals were classified into two distinct categories: signals that were higher for adults than for larvae of H. contortus (n 2022-01-01T00:00:00Z2022 Jul2026-05-28T04:04:47.728Z2025-02-19T03:29:33.314ZS-EPMC93386263590789210.1186/s13071-022-05400-w