biostudies-literatureUnknown31(9)Liu KThe study aimed to characterize phenolic compounds of the <i>Inonotus sanghuang</i>'s ethyl-acetate fraction (EAF) and assess the neuroprotective effect of EAF using the H₂O₂-treated primary cortical neuronal cells (PCNC) model. Using HPLC-ECD, 5 phenolics were identified and quantified from EAF. H₂O₂-treated PCNC experiments in vitro showed that pretreatment with EAF increased the GSH-PX and SOD activities and reduced the NO, MDA, and Aβ contents. Furthermore, EAF suppressed the production of IL-1β, IFN-γ, IL-6, and TNF-α in H₂O₂-treated PCNC. Other mechanisms found that EAF reduced Bax, caspase 9, and caspase 3 expressions at the mRNA and protein levels while increasing Bcl-2 expression at the mRNA and protein levels. ThesFood science and biotechnology1213-1223https://www.ebi.ac.uk/biostudies/studies/S-EPMC9339070biostudies-literaturePhenolic composition and neuroprotective effects of the ethyl-acetate fraction from <i>Inonotus sanghuang</i> against H₂O₂-induced apoptotic cell death of primary cortical neuronal cells.PMC9339070Liu YLiu KHuai YHu HXiao XQi CWang JfalsePhenolic composition and neuroprotective effects of the ethyl-acetate fraction from <i>Inonotus sanghuang</i> against H₂O₂-induced apoptotic cell death of primary cortical neuronal cells.The study aimed to characterize phenolic compounds of the <i>Inonotus sanghuang</i>'s ethyl-acetate fraction (EAF) and assess the neuroprotective effect of EAF using the H₂O₂-treated primary cortical neuronal cells (PCNC) model. Using HPLC-ECD, 5 phenolics were identified and quantified from EAF. H₂O₂-treated PCNC experiments in vitro showed that pretreatment with EAF increased the GSH-PX and SOD activities and reduced the NO, MDA, and Aβ contents. Furthermore, EAF suppressed the production of IL-1β, IFN-γ, IL-6, and TNF-α in H₂O₂-treated PCNC. Other mechanisms found that EAF reduced Bax, caspase 9, and caspase 3 expressions at the mRNA and protein levels while increasing Bcl-2 expression at the mRNA and protein levels. Thes2022-01-01T00:00:00Z2022 Aug2025-04-04T18:42:03.596Z2025-04-04T18:42:03.596ZS-EPMC93390703591935510.1007/s10068-022-01107-x