biostudies-literatureYang XNIDDK NIH HHSAmerican Diabetes AssociationNational Institutes of Healthdev200432https://www.ebi.ac.uk/biostudies/studies/S-EPMC9340549biostudies-literatureUnknown149(13)Pancreatic and duodenal homeobox 1 (PDX1) is crucial for pancreas organogenesis, yet the dynamic changes in PDX1 binding in human or mouse developing pancreas have not been examined. To address this knowledge gap, we performed PDX1 ChIP-seq and single-cell RNA-seq using fetal human pancreata. We integrated our datasets with published datasets and revealed the dynamics of PDX1 binding and potential cell lineage-specific PDX1-bound genes in the pancreas from fetal to adult stages. We identified a core set of developmentally conserved PDX1-bound genes that reveal the broad multifaceted role of PDX1 in pancreas development. Despite the well-known dramatic changes in PDX1 function and expression, we found that PDX1-bound genes are largely conserved from embryonic to adult stages. This points toDevelopment (Cambridge, England)A PDX1 cistrome and single-cell transcriptome resource of the developing pancreas.PMC9340549R01DK121175P30 DK019525F32-DK-089747R01 DK105689R01DK1056891-16-JDF086R01 DK121175F32 DK089747Yang XYang JRice GYu RLi CStoffers DARaum JCKim JStanescu DEWon KJfalseA PDX1 cistrome and single-cell transcriptome resource of the developing pancreas.Pancreatic and duodenal homeobox 1 (PDX1) is crucial for pancreas organogenesis, yet the dynamic changes in PDX1 binding in human or mouse developing pancreas have not been examined. To address this knowledge gap, we performed PDX1 ChIP-seq and single-cell RNA-seq using fetal human pancreata. We integrated our datasets with published datasets and revealed the dynamics of PDX1 binding and potential cell lineage-specific PDX1-bound genes in the pancreas from fetal to adult stages. We identified a core set of developmentally conserved PDX1-bound genes that reveal the broad multifaceted role of PDX1 in pancreas development. Despite the well-known dramatic changes in PDX1 function and expression, we found that PDX1-bound genes are largely conserved from embryonic to adult stages. This points to2022-01-01T00:00:00Z2022 Jul2026-05-09T13:59:38.95Z2025-04-04T20:28:29.923ZS-EPMC93405493570834910.1242/dev.200432