biostudies-literatureOmenn GSKorean Ministry of Health and WelfareNIA NIH HHSNIEHS NIH HHSFarmer Family FoundationCanadian institutes of health research foundationNCI NIH HHSNational Health and Medical Research CouncilKnut och Alice Wallenbergs StiftelseNational Institutes of HealthSwiss Institute of BioinformaticsNIGMS NIH HHSNational Science Foundation5227-5240https://www.ebi.ac.uk/biostudies/studies/S-EPMC9340669biostudies-literatureUnknown20(12)The 2021 Metrics of the HUPO Human Proteome Project (HPP) show that protein expression has now been credibly detected (neXtProt PE1 level) for 18 357 (92.8%) of the 19 778 predicted proteins coded in the human genome, a gain of 483 since 2020 from reports throughout the world reanalyzed by the HPP. Conversely, the number of neXtProt PE2, PE3, and PE4 missing proteins has been reduced by 478 to 1421. This represents remarkable progress on the proteome parts list. The utilization of proteomics in a broad array of biological and clinical studies likewise continues to expand with many important findings and effective integration with other omics platforms. We present highlights from the Immunopeptidomics, Glycoproteomics, Infectious Disease, Cardiovascular, Musculo-Skeletal, Liver, and CancersJournal of proteome researchProgress Identifying and Analyzing the Human Proteome: 2021 Metrics from the HUPO Human Proteome Project.PMC9340669HI13C22098U19AG023122U01 CA253217P30 CA008748R24 GM127667DBI-1933311P30ES017885-01A1U24 CA210967R24GM127667P30 ES017885U24CA21096717X173R21CA251992R21 CA263262R01 GM087221R01GM114141R13 CA135997R01 GM114141R01GM087221148408HI16C0257P30CA008748R21 CA251992R21CA263262U01CA253217U19 AG023122APP1010303Corrales FJLane LLindskog CDeutsch EWCristea IMOverall CMLiu SPaik YKSrivastava SBandeira NAebersold RChen YJOmenn GSRoehrl MHAWeintraub SMoritz RLfalseProgress Identifying and Analyzing the Human Proteome: 2021 Metrics from the HUPO Human Proteome Project.The 2021 Metrics of the HUPO Human Proteome Project (HPP) show that protein expression has now been credibly detected (neXtProt PE1 level) for 18 357 (92.8%) of the 19 778 predicted proteins coded in the human genome, a gain of 483 since 2020 from reports throughout the world reanalyzed by the HPP. Conversely, the number of neXtProt PE2, PE3, and PE4 missing proteins has been reduced by 478 to 1421. This represents remarkable progress on the proteome parts list. The utilization of proteomics in a broad array of biological and clinical studies likewise continues to expand with many important findings and effective integration with other omics platforms. We present highlights from the Immunopeptidomics, Glycoproteomics, Infectious Disease, Cardiovascular, Musculo-Skeletal, Liver, and Cancers2021-01-01T00:00:00Z2021 Dec2025-04-21T22:28:49.622Z2025-04-05T18:48:27.043ZS-EPMC93406693467009210.1021/acs.jproteome.1c00590