{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Li L"],"funding":["Central South University","Ministry of Science and Technology of the People's Republic of China","Hunan Provincial Science and Technology Department","Ministry of Science and Technology of the People's Republic of China","National Natural Science Foundation of China"],"pagination":["25"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9343540"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(1)"],"pubmed_abstract":["Mouse embryonic stem cells (mESCs) cycle in and out of a transient 2-cell (2C)-like totipotent state, driven by a complex genetic circuit involves both the coding and repetitive sections of the genome. While a vast array of regulators, including the multi-functional protein Rif1, has been reported to influence the switch of fate potential, how they act in concert to achieve this cellular plasticity remains elusive. Here, by modularizing the known totipotency regulatory factors, we identify an unprecedented functional connection between Rif1 and the non-canonical polycomb repressive complex PRC1.6. Downregulation of the expression of either Rif1 or PRC1.6 subunits imposes similar impacts on the transcriptome of mESCs. The LacO-LacI induced ectopic colocalization assay detects a specific int"],"journal":["Cell regeneration (London, England)"],"pubmed_title":["Rif1 interacts with non-canonical polycomb repressive complex PRC1.6 to regulate mouse embryonic stem cells fate potential."],"pmcid":["PMC9343540"],"funding_grant_id":["2018CX032, 2020CX016","2019JJ40478","2021YFC2701202","32170821, 31771589","2021JJ10054, 2019SK1012, 2018DK2015, 2017RS3013, 2017XK2011","2019RS1010"],"pubmed_authors":["Liu J","Lv L","Mao S","Chen J","Zhu Y","Hu G","Li P","Chen F","Yuan K","Shen Y","Li L"],"additional_accession":[]},"is_claimable":false,"name":"Rif1 interacts with non-canonical polycomb repressive complex PRC1.6 to regulate mouse embryonic stem cells fate potential.","description":"Mouse embryonic stem cells (mESCs) cycle in and out of a transient 2-cell (2C)-like totipotent state, driven by a complex genetic circuit involves both the coding and repetitive sections of the genome. While a vast array of regulators, including the multi-functional protein Rif1, has been reported to influence the switch of fate potential, how they act in concert to achieve this cellular plasticity remains elusive. Here, by modularizing the known totipotency regulatory factors, we identify an unprecedented functional connection between Rif1 and the non-canonical polycomb repressive complex PRC1.6. Downregulation of the expression of either Rif1 or PRC1.6 subunits imposes similar impacts on the transcriptome of mESCs. The LacO-LacI induced ectopic colocalization assay detects a specific int","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Aug","modification":"2026-04-08T18:26:05.372Z","creation":"2025-02-19T01:29:05.244Z"},"accession":"S-EPMC9343540","cross_references":{"pubmed":["35915272"],"doi":["10.1186/s13619-022-00124-9"]}}