{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["5(3)"],"submitter":["Renner P"],"pubmed_abstract":["Abstract <h4>Background</h4> Antibody-secreting cells are terminally differentiated B cells that play a critical role in humoral immunity through immunoglobulin secretion along with possessing the potential to be long-lived. It is now appreciated that ASCs regulate multiple aspects of biology through the secretion of various cytokines. In this regard, ICFC is a key tool used to assess the presence of intracellular proteins such as cytokines and transcription factors. <h4>Methods</h4> Paraformaldehyde plus saponin or the eBioscience Foxp3/Transcription Factor Staining Buffer Set were used to evaluate the non-specific intracellular retention of phycoerythrin-containing antibody conjugates by ASCs. <h4>Results</h4> We showed that the use of phycoerythrin-containing antibody conjugates led to "],"journal":["Antibody therapeutics"],"pagination":["151-163"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9344851"],"repository":["biostudies-literature"],"pubmed_title":["Intracellular flow cytometry staining of antibody-secreting cells using phycoerythrin-conjugated antibodies: pitfalls and solutions"],"pmcid":["PMC9344851"],"pubmed_authors":["Pioli K","Renner P","Crone M","Pioli P","Kornas M"],"additional_accession":[]},"is_claimable":false,"name":"Intracellular flow cytometry staining of antibody-secreting cells using phycoerythrin-conjugated antibodies: pitfalls and solutions","description":"Abstract <h4>Background</h4> Antibody-secreting cells are terminally differentiated B cells that play a critical role in humoral immunity through immunoglobulin secretion along with possessing the potential to be long-lived. It is now appreciated that ASCs regulate multiple aspects of biology through the secretion of various cytokines. In this regard, ICFC is a key tool used to assess the presence of intracellular proteins such as cytokines and transcription factors. <h4>Methods</h4> Paraformaldehyde plus saponin or the eBioscience Foxp3/Transcription Factor Staining Buffer Set were used to evaluate the non-specific intracellular retention of phycoerythrin-containing antibody conjugates by ASCs. <h4>Results</h4> We showed that the use of phycoerythrin-containing antibody conjugates led to ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jun","modification":"2025-04-18T13:15:43.31Z","creation":"2025-02-19T05:06:02.125Z"},"accession":"S-EPMC9344851","cross_references":{}}