biostudies-literatureUnknown5(3)Renner PAbstract <h4>Background</h4> Antibody-secreting cells are terminally differentiated B cells that play a critical role in humoral immunity through immunoglobulin secretion along with possessing the potential to be long-lived. It is now appreciated that ASCs regulate multiple aspects of biology through the secretion of various cytokines. In this regard, ICFC is a key tool used to assess the presence of intracellular proteins such as cytokines and transcription factors. <h4>Methods</h4> Paraformaldehyde plus saponin or the eBioscience Foxp3/Transcription Factor Staining Buffer Set were used to evaluate the non-specific intracellular retention of phycoerythrin-containing antibody conjugates by ASCs. <h4>Results</h4> We showed that the use of phycoerythrin-containing antibody conjugates led to Antibody therapeutics151-163https://www.ebi.ac.uk/biostudies/studies/S-EPMC9344851biostudies-literatureIntracellular flow cytometry staining of antibody-secreting cells using phycoerythrin-conjugated antibodies: pitfalls and solutionsPMC9344851Pioli KRenner PCrone MPioli PKornas MfalseIntracellular flow cytometry staining of antibody-secreting cells using phycoerythrin-conjugated antibodies: pitfalls and solutionsAbstract <h4>Background</h4> Antibody-secreting cells are terminally differentiated B cells that play a critical role in humoral immunity through immunoglobulin secretion along with possessing the potential to be long-lived. It is now appreciated that ASCs regulate multiple aspects of biology through the secretion of various cytokines. In this regard, ICFC is a key tool used to assess the presence of intracellular proteins such as cytokines and transcription factors. <h4>Methods</h4> Paraformaldehyde plus saponin or the eBioscience Foxp3/Transcription Factor Staining Buffer Set were used to evaluate the non-specific intracellular retention of phycoerythrin-containing antibody conjugates by ASCs. <h4>Results</h4> We showed that the use of phycoerythrin-containing antibody conjugates led to 2022-01-01T00:00:00Z2022 Jun2025-04-18T13:15:43.31Z2025-02-19T05:06:02.125ZS-EPMC9344851