biostudies-literatureElomaa HSyöpäsäätiöNCI NIH HHSU.S. Department of Health &amp; Human Services | National Institutes of Health514-523https://www.ebi.ac.uk/biostudies/studies/S-EPMC9345858biostudies-literatureUnknown127(3)<h4>Background</h4>Although high T cell density is a strong favourable prognostic factor in colorectal cancer, the significance of the spatial distribution of T cells is incompletely understood. We aimed to evaluate the prognostic significance of tumour cell-T cell co-localisation and T cell densities.<h4>Methods</h4>We analysed CD3 and CD8 immunohistochemistry in a study cohort of 983 colorectal cancer patients and a validation cohort (N = 246). Individual immune and tumour cells were identified to calculate T cell densities (to derive T cell density score) and G-cross function values, estimating the likelihood of tumour cells being co-located with T cells within 20 µm radius (to derive T cell proximity score).<h4>Results</h4>High T cell proximity score associated with longer cancer-specific survival in both the study cohort [adjusted HR for high (vs. low) 0.33, 95% CI 0.20-0.52, P_trend < 0.0001] and the validation cohort [adjusted HR for high (vs. low) 0.15, 95% CI 0.05-0.45, P_trend < 0.0001] and its prognostic value was independent of T cell density score.<h4>Conclusions</h4>The spatial point pattern analysis of tumour cell-T cell co-localisation could provide detailed information on colorectal cancer prognosis, supporting the value of spatial measurement of T cell infiltrates as a novel, robust tumour-immune biomarker.British journal of cancerPrognostic significance of spatial and density analysis of T lymphocytes in colorectal cancer.PMC9345858R35 CA19773559-5619Vayrynen SAHelminen OElomaa HWirta EVAhtiainen MVayrynen JPSeppala TTOgino SBohm JMakinen MJKuopio TFriman MMecklin JPNowak JAfalsePrognostic significance of spatial and density analysis of T lymphocytes in colorectal cancer.<h4>Background</h4>Although high T cell density is a strong favourable prognostic factor in colorectal cancer, the significance of the spatial distribution of T cells is incompletely understood. We aimed to evaluate the prognostic significance of tumour cell-T cell co-localisation and T cell densities.<h4>Methods</h4>We analysed CD3 and CD8 immunohistochemistry in a study cohort of 983 colorectal cancer patients and a validation cohort (N = 246). Individual immune and tumour cells were identified to calculate T cell densities (to derive T cell density score) and G-cross function values, estimating the likelihood of tumour cells being co-located with T cells within 20 µm radius (to derive T cell proximity score).<h4>Results</h4>High T cell proximity score associated with longer cancer-specific survival in both the study cohort [adjusted HR for high (vs. low) 0.33, 95% CI 0.20-0.52, P_trend < 0.0001] and the validation cohort [adjusted HR for high (vs. low) 0.15, 95% CI 0.05-0.45, P_trend < 0.0001] and its prognostic value was independent of T cell density score.<h4>Conclusions</h4>The spatial point pattern analysis of tumour cell-T cell co-localisation could provide detailed information on colorectal cancer prognosis, supporting the value of spatial measurement of T cell infiltrates as a novel, robust tumour-immune biomarker.2022-01-01T00:00:00Z2022 Aug2024-11-15T22:36:51.191Z2024-11-15T22:36:51.191ZS-EPMC93458583544945310.1038/s41416-022-01822-6