biostudies-literatureYang HU.S. Department of Health &amp; Human Services | NIH | National Institute of Neurological Disorders and StrokeU.S. Department of Health &amp; Human Services | NIH | National Cancer InstituteNHLBI NIH HHSU.S. Department of Health &amp; Human Services | NIH | National Heart, Lung, and Blood InstituteNCI NIH HHSNINDS NIH HHS422-435https://www.ebi.ac.uk/biostudies/studies/S-EPMC9345892biostudies-literatureUnknown127(3)<h4>Background</h4>Angiogenin is a multifunctional secreted ribonuclease that is upregulated in human cancers and downregulated or mutationally inactivated in neurodegenerative diseases. A role for angiogenin in glioblastoma was inferred from the inverse correlation of angiogenin expression with patient survival but had not been experimentally investigated.<h4>Methods</h4>Angiogenin knockout mice were generated and the effect of angiogenin deficiency on glioblastoma progression was examined. Angiogenin and plexin-B2 genes were knocked down in glioblastoma cells and the changes in cell proliferation, invasion and vascular association were examined. Monoclonal antibodies of angiogenin and small molecules were used to assess the therapeutic activity of the angiogenin-plexin-B2 pathway in bothBritish journal of cancerAngiogenin and plexin-B2 axis promotes glioblastoma progression by enhancing invasion, vascular association, proliferation and survival.PMC9345892R01 HL135160R01CA105241R01NS065237R01 CA105241R01 NS065237R01HL135160Yang HJay DYuan LIbaragi SVanli NJiang YHu AJCochran BLi SKishikawa HShapiro RHolland ECYu WGoncalves KAHu GFfalseAngiogenin and plexin-B2 axis promotes glioblastoma progression by enhancing invasion, vascular association, proliferation and survival.<h4>Background</h4>Angiogenin is a multifunctional secreted ribonuclease that is upregulated in human cancers and downregulated or mutationally inactivated in neurodegenerative diseases. A role for angiogenin in glioblastoma was inferred from the inverse correlation of angiogenin expression with patient survival but had not been experimentally investigated.<h4>Methods</h4>Angiogenin knockout mice were generated and the effect of angiogenin deficiency on glioblastoma progression was examined. Angiogenin and plexin-B2 genes were knocked down in glioblastoma cells and the changes in cell proliferation, invasion and vascular association were examined. Monoclonal antibodies of angiogenin and small molecules were used to assess the therapeutic activity of the angiogenin-plexin-B2 pathway in both2022-01-01T00:00:00Z2022 Aug2025-05-29T19:43:26.595Z2025-05-29T19:43:26.595ZS-EPMC93458923541821210.1038/s41416-022-01814-6