biostudies-literatureUnknown13Yuanyuan NBacteriophages (phages) are capable of infecting specific bacteria, and therefore can be used as a biological control agent to control bacteria-induced animal, plant, and human diseases. In this study, two homolog phages (named PPAY and PPAT) that infect <i>Pseudomonas aeruginosa</i> PAO1 were isolated and characterized. The results of the phage plaque assay showed that PPAT plaques were transparent dots, while the PPAY plaques were translucent dots with a halo. Transmission electron microscopy results showed that PPAT (65 nm) and PPAY (60 nm) strains are similar in size and have an icosahedral head and a short tail. Therefore, these belong to the short-tailed phage family <i>Podoviridae</i>. One-step growth curves revealed the latent period of 20 min and burst time of 30 min for PPAT and Frontiers in microbiology946251https://www.ebi.ac.uk/biostudies/studies/S-EPMC9348578biostudies-literatureIsolation and characterization of two homolog phages infecting <i>Pseudomonas aeruginosa</i>.PMC9348578Chenyu LXi ZYun LXiaobo YYuanyuan NJingfeng WYutong YZhiqiang SBin XHongrui ZChen ZPingfeng YZhigang QShang WfalseIsolation and characterization of two homolog phages infecting <i>Pseudomonas aeruginosa</i>.Bacteriophages (phages) are capable of infecting specific bacteria, and therefore can be used as a biological control agent to control bacteria-induced animal, plant, and human diseases. In this study, two homolog phages (named PPAY and PPAT) that infect <i>Pseudomonas aeruginosa</i> PAO1 were isolated and characterized. The results of the phage plaque assay showed that PPAT plaques were transparent dots, while the PPAY plaques were translucent dots with a halo. Transmission electron microscopy results showed that PPAT (65 nm) and PPAY (60 nm) strains are similar in size and have an icosahedral head and a short tail. Therefore, these belong to the short-tailed phage family <i>Podoviridae</i>. One-step growth curves revealed the latent period of 20 min and burst time of 30 min for PPAT and 2022-01-01T00:00:00Z20222026-06-01T02:18:17.228Z2025-04-05T12:11:52.822ZS-EPMC93485783593519710.3389/fmicb.2022.946251