biostudies-literatureFeng JLNational Science &amp; Technology Fundamental Resources Investigation Program of China2194021https://www.ebi.ac.uk/biostudies/studies/S-EPMC9348943biostudies-literatureUnknown2022<i>Iris</i> species, commonly known as rainbow flowers because of their attractive flowers, are extensively grown in landscape gardens. A few species, including <i>Belamcanda chinensis</i>, the synonym of <i>I. domestica</i> and <i>I. tectorum</i>, are known for their medicinal properties. However, research on the genomes and evolutionary relationships of <i>Iris</i> species is scarce. In the current study, the complete chloroplast (CP) genomes of <i>I. tectorum</i>, <i>I. dichotoma</i>, <i>I. japonica</i>, and <i>I. domestica</i> were sequenced and compared for their identification and relationship. The CP genomes of the four <i>Iris</i> species were circular quadripartite with similar lengths, GC contents, and codon usages. A total of 113 specific genes were annotated, including the <i>yBioMed research internationalComparison Analysis Based on Complete Chloroplast Genomes and Insights into Plastid Phylogenomic of Four <i>Iris</i> Species.PMC93489432018FY100700Feng JLWu LWLi BLWang QLin YLPan YJYao HfalseComparison Analysis Based on Complete Chloroplast Genomes and Insights into Plastid Phylogenomic of Four <i>Iris</i> Species.<i>Iris</i> species, commonly known as rainbow flowers because of their attractive flowers, are extensively grown in landscape gardens. A few species, including <i>Belamcanda chinensis</i>, the synonym of <i>I. domestica</i> and <i>I. tectorum</i>, are known for their medicinal properties. However, research on the genomes and evolutionary relationships of <i>Iris</i> species is scarce. In the current study, the complete chloroplast (CP) genomes of <i>I. tectorum</i>, <i>I. dichotoma</i>, <i>I. japonica</i>, and <i>I. domestica</i> were sequenced and compared for their identification and relationship. The CP genomes of the four <i>Iris</i> species were circular quadripartite with similar lengths, GC contents, and codon usages. A total of 113 specific genes were annotated, including the <i>y2022-01-01T00:00:00Z20222026-05-28T00:38:49.794Z2025-04-22T16:34:44.673ZS-EPMC93489433593741210.1155/2022/2194021