biostudies-literatureUnknown28 Suppl 2Travis BJMidwestern University Department of Microbiology & ImmunologyMidwestern University College of Dental Medicine<h4>Objective</h4>This work aims to determine the efficacy of preprocedural oral rinsing with chlorine dioxide solutions to minimize the risk of coronavirus disease 2019 (COVID-19) transmission during high-risk dental procedures.<h4>Methods</h4>The antiviral activity of chlorine-dioxide-based oral rinse (OR) solutions was tested by pre-incubating with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pseudovirus in a dosage-dependent manner before transducing to human embryonic kidney epithelial (HEK293T-ACE2) cells, which stably expresses ACE-2 receptor. Viral entry was determined by measuring luciferase activity using a luminescence microplate reader. In the cell-to-cell fusion assay, effector Chinese hamster ovary (CHO-K1) cells co-expressing spike glycoprotein of SARS-CoV-2 Oral diseases2481-2491https://www.ebi.ac.uk/biostudies/studies/S-EPMC9349900biostudies-literatureSignificance of chlorine-dioxide-based oral rinses in preventing SARS-CoV-2 cell entry.PMC9349900Kohlmeir ETiwari VElste JTravis BJMitchell JCJoo BYGao FCuevas-Nunez MfalseSignificance of chlorine-dioxide-based oral rinses in preventing SARS-CoV-2 cell entry.<h4>Objective</h4>This work aims to determine the efficacy of preprocedural oral rinsing with chlorine dioxide solutions to minimize the risk of coronavirus disease 2019 (COVID-19) transmission during high-risk dental procedures.<h4>Methods</h4>The antiviral activity of chlorine-dioxide-based oral rinse (OR) solutions was tested by pre-incubating with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pseudovirus in a dosage-dependent manner before transducing to human embryonic kidney epithelial (HEK293T-ACE2) cells, which stably expresses ACE-2 receptor. Viral entry was determined by measuring luciferase activity using a luminescence microplate reader. In the cell-to-cell fusion assay, effector Chinese hamster ovary (CHO-K1) cells co-expressing spike glycoprotein of SARS-CoV-2 2022-01-01T00:00:00Z2022 Nov2025-04-19T22:57:18.693Z2025-02-19T04:54:00.82ZS-EPMC93499003584137710.1111/odi.14319