biostudies-literatureMartin-Otal CCARAMBAAUTOCARTPaula &amp; Rodger Riney FoundationEuropean UnionMinisterio de Ciencia e InnovaciónHepacare ProjectGobierno de Navarra IndustriaFundación Bancaria La Caixae004479https://www.ebi.ac.uk/biostudies/studies/S-EPMC9351345biostudies-literatureUnknown10(8)<h4>Background</h4>One of the main difficulties of adoptive cell therapies with chimeric antigen receptor (CAR)-T cells in solid tumors is the identification of specific target antigens. The tumor microenvironment can present suitable antigens for CAR design, even though they are not expressed by the tumor cells. We have generated a CAR specific for the splice variant extra domain A (EDA) of fibronectin, which is highly expressed in the tumor stroma of many types of tumors but not in healthy tissues.<h4>Methods</h4>EDA expression was explored in RNA-seq data from different human tumor types and by immunohistochemistry in paraffin-embedded tumor biopsies. Murine and human anti-EDA CAR-T cells were prepared using recombinant retro/lentiviruses, respectively. The functionality of EDA CAR-T ceJournal for immunotherapy of cancerTargeting the extra domain A of fibronectin for cancer therapy with CAR-T cells.PMC9351345H2020-JTI-IMI2-2019-18T2EVOLVEMCIN/AEI/10.13039945393IJCI-2017-34204PID2021-128283OA-I00SC1-PM-08-20170011-1411-2019-000079, DESCARTHeSPLEC2021-008094PID2019-108989RB-I000011-1411-2022-000088, SOCRATHeS501100011033RTC-2017-6585-1754658Conde ECasares NDe Andrea CEHervas-Stubbs SGorraiz MMartin-Otal CSarrion PLasarte-Cia AVilas AProsper FSan Miguel JSerrano DEcheveste JCalvo ALasarte JJLozano TNavarro FRodriguez-Madoz JRSanchez-Moreno IfalseTargeting the extra domain A of fibronectin for cancer therapy with CAR-T cells.<h4>Background</h4>One of the main difficulties of adoptive cell therapies with chimeric antigen receptor (CAR)-T cells in solid tumors is the identification of specific target antigens. The tumor microenvironment can present suitable antigens for CAR design, even though they are not expressed by the tumor cells. We have generated a CAR specific for the splice variant extra domain A (EDA) of fibronectin, which is highly expressed in the tumor stroma of many types of tumors but not in healthy tissues.<h4>Methods</h4>EDA expression was explored in RNA-seq data from different human tumor types and by immunohistochemistry in paraffin-embedded tumor biopsies. Murine and human anti-EDA CAR-T cells were prepared using recombinant retro/lentiviruses, respectively. The functionality of EDA CAR-T ce2022-01-01T00:00:00Z2022 Aug2026-07-14T17:15:59.513Z2026-06-21T03:13:07.408ZS-EPMC93513453591812310.1136/jitc-2021-004479