{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Cevallos-Cedeno RE"],"funding":["Subsecretar?a de Educaci?n Superior","Secretar?a de Educaci?n Superior, Ciencia, Tecnolog?a e Innovaci?n","European Regional Development Fund","Ministerio de Econom?a y Competitividad"],"pagination":["10857-10864"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9352146"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["94(30)"],"pubmed_abstract":["Algal blooms that contaminate freshwater resources with cyanotoxins constitute, nowadays, a global concern. To deal with this problem, a variety of analytical methods, including immunochemical assays, are available for the main algal toxins, for example, microcystins, nodularins, and saxitoxins, with the remarkable exception of anatoxin-a. Now, for the first time, highly sensitive, enantioselective immunoassays for anatoxin-a have been validated using homemade monoclonal antibodies. Two competitive enzyme-linked immunosorbent assays were developed in different formats, with detection limits for (+)-anatoxin-a of 0.1 ng/mL. Excellent recovery values between 82 and 117%, and coefficients of variation below 20%, were observed using environmental water samples fortified between 0.5 and 500 ng/mL. In addition, a lateral-flow immunochromatographic assay was optimized for visual and instrumental reading of results. This test showed a visual detection limit for (+)-anatoxin-a of 4 ng/mL. Performance with a reader was validated in accordance with the European guidelines for semiquantitative rapid methods for small chemical contaminants. Thus, at a screening target concentration of 2 ng/mL, the probability of a blank sample to be classified as \"suspect\" was as low as 0.2%. Finally, the optimized direct enzyme immunoassay was validated by comparison with high-performance liquid chromatography-tandem mass spectroscopy data and showed a good correlation (<i>r</i> = 0.995) with a slope of 0.94. Moreover, environmental water samples containing more than 2 ng/mL of anatoxin-a were detected by the developed dipstick assay. These results provide supplementary and complementary strategies for monitoring the presence of anatoxin-a in water."],"journal":["Analytical chemistry"],"pubmed_title":["Rapid Immunochemical Methods for Anatoxin-a Monitoring in Environmental Water Samples."],"pmcid":["PMC9352146"],"funding_grant_id":["RTI2018-096121-B-C21/22","AGL2015-64488-C2-1/2-R"],"pubmed_authors":["Abad-Fuentes A","Abad-Somovilla A","Mercader JV","Quinones-Reyes G","Agullo C","Cevallos-Cedeno RE"],"additional_accession":[]},"is_claimable":false,"name":"Rapid Immunochemical Methods for Anatoxin-a Monitoring in Environmental Water Samples.","description":"Algal blooms that contaminate freshwater resources with cyanotoxins constitute, nowadays, a global concern. To deal with this problem, a variety of analytical methods, including immunochemical assays, are available for the main algal toxins, for example, microcystins, nodularins, and saxitoxins, with the remarkable exception of anatoxin-a. Now, for the first time, highly sensitive, enantioselective immunoassays for anatoxin-a have been validated using homemade monoclonal antibodies. Two competitive enzyme-linked immunosorbent assays were developed in different formats, with detection limits for (+)-anatoxin-a of 0.1 ng/mL. Excellent recovery values between 82 and 117%, and coefficients of variation below 20%, were observed using environmental water samples fortified between 0.5 and 500 ng/mL. In addition, a lateral-flow immunochromatographic assay was optimized for visual and instrumental reading of results. This test showed a visual detection limit for (+)-anatoxin-a of 4 ng/mL. Performance with a reader was validated in accordance with the European guidelines for semiquantitative rapid methods for small chemical contaminants. Thus, at a screening target concentration of 2 ng/mL, the probability of a blank sample to be classified as \"suspect\" was as low as 0.2%. Finally, the optimized direct enzyme immunoassay was validated by comparison with high-performance liquid chromatography-tandem mass spectroscopy data and showed a good correlation (<i>r</i> = 0.995) with a slope of 0.94. Moreover, environmental water samples containing more than 2 ng/mL of anatoxin-a were detected by the developed dipstick assay. These results provide supplementary and complementary strategies for monitoring the presence of anatoxin-a in water.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Aug","modification":"2026-03-31T11:21:06.838Z","creation":"2025-02-19T04:54:19.438Z"},"accession":"S-EPMC9352146","cross_references":{"pubmed":["35853613"],"doi":["10.1021/acs.analchem.2c01939"]}}