biostudies-literatureZhou JNGuangzhou Health Care and Cooperative Innovation Major ProjectNational Natural Science Foundation of ChinaNational Science and Technology Major Projects for "Major New Drugs Innovation and Development"National Key Research and Development Program of Chinae2201166https://www.ebi.ac.uk/biostudies/studies/S-EPMC9353477biostudies-literatureUnknown9(22)Cancer stem cells (CSCs) are reported to play essential roles in chemoresistance and metastasis. Pathways regulating CSC self-renewal and proliferation, such as Hedgehog, Notch, Wnt/β-catenin, TGF-β, and Myc, may be potential therapeutic targets. Here, a functional screening from the focused library with 365 compounds is performed by a step-by-step strategy. Among these candidate molecules, phenyl-2-pyrimidinyl ketone 4-allyl-3-amino selenourea (CU27) is chosen for further identification because it proves to be the most effective compound over others on CSC inhibition. Through ingenuity pathway analysis, it is shown CU27 may inhibit CSC through a well-known stemness-related transcription factor c-Myc. Gene set enrichment analysis, dual-luciferase reporter assays, expression levels of typicAdvanced science (Weinheim, Baden-Wurttemberg, Germany)A Functional Screening Identifies a New Organic Selenium Compound Targeting Cancer Stem Cells: Role of c-Myc Transcription Activity Inhibition in Liver Cancer.PMC93534772017YFA01031042017YFA0103103817731372017YFA01031002018ZX09711003201704020224Xu YCZhang MMZeng QXi JFHe LJZhong WZhou JNZhang MJia YLNan XPei XTZhou XBWang HYFan SYWang DXWang XKLi SYue WZhang BfalseA Functional Screening Identifies a New Organic Selenium Compound Targeting Cancer Stem Cells: Role of c-Myc Transcription Activity Inhibition in Liver Cancer.Cancer stem cells (CSCs) are reported to play essential roles in chemoresistance and metastasis. Pathways regulating CSC self-renewal and proliferation, such as Hedgehog, Notch, Wnt/β-catenin, TGF-β, and Myc, may be potential therapeutic targets. Here, a functional screening from the focused library with 365 compounds is performed by a step-by-step strategy. Among these candidate molecules, phenyl-2-pyrimidinyl ketone 4-allyl-3-amino selenourea (CU27) is chosen for further identification because it proves to be the most effective compound over others on CSC inhibition. Through ingenuity pathway analysis, it is shown CU27 may inhibit CSC through a well-known stemness-related transcription factor c-Myc. Gene set enrichment analysis, dual-luciferase reporter assays, expression levels of typic2022-01-01T00:00:00Z2022 Aug2026-05-10T06:25:06.261Z2024-11-09T19:32:18.539ZS-EPMC93534773565226410.1002/advs.202201166