{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["Li P"],"pubmed_abstract":["Gray mold caused by <i>Botrytis cinerea</i> is detrimental to plants and fruits. Endophytes have been shown to modify plant disease severity in functional assays. We conducted this study to investigate the endophytic strain <i>Bacillus</i> K1 with excellently antagonistic <i>B. cinerea</i> from the wild grape endosphere. We identified a wild grape endophytic strain K1 with high antifungal activity against <i>B. cinerea</i> both <i>in vitro</i> and <i>in vivo</i>. Combining the phylogenetic results based on 16S rDNA and genome sequencing, K1 was assigned as <i>Bacillus subtilis</i>. The <i>in vitro</i> results displayed that K1 and its volatile substances could significantly inhibit the mycelia growth of <i>B. cinerea</i>. Grape fruit inoculated with <i>Bacillus</i> K1 showed lower gray mold during treatment. The higher levels of defense-related enzymes, including peroxidase, polyphenol oxidase, and phenylalanine ammonia lyase, were induced in grapes after inoculation. Scanning electron microscopy (SEM) suggested that K1 inhibited mycelial growth <i>via</i> bacterial colonization and antibiosis in grapes. The gas chromatography-mass spectrometry analysis identified 33 volatiles in which dibutyl phthalate was the major compound accounting for 74.28%. Dibutyl phthalate demonstrated strong activity in suppressing the mycelia growth of <i>B. cinerea</i>. Genome bioinformatics analysis revealed that the K1 chromosome harbored many known biosynthesis gene clusters encoding subtilosin, bacillaene, bacillibactin, bacilysin, and fengycin. This study provides a potential biological agent to control diseases of post-harvest grape fruit and improves our understanding of the possible biocontrol mechanisms of the <i>Bacillus</i> strain."],"journal":["Frontiers in microbiology"],"pagination":["935675"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9355035"],"repository":["biostudies-literature"],"pubmed_title":["Antifungal Activity of Endophytic <i>Bacillus</i> K1 Against <i>Botrytis cinerea</i>."],"pmcid":["PMC9355035"],"pubmed_authors":["Yao Z","Wei B","Shi S","Li P","Feng B","Zhao Y"],"additional_accession":[]},"is_claimable":false,"name":"Antifungal Activity of Endophytic <i>Bacillus</i> K1 Against <i>Botrytis cinerea</i>.","description":"Gray mold caused by <i>Botrytis cinerea</i> is detrimental to plants and fruits. Endophytes have been shown to modify plant disease severity in functional assays. We conducted this study to investigate the endophytic strain <i>Bacillus</i> K1 with excellently antagonistic <i>B. cinerea</i> from the wild grape endosphere. We identified a wild grape endophytic strain K1 with high antifungal activity against <i>B. cinerea</i> both <i>in vitro</i> and <i>in vivo</i>. Combining the phylogenetic results based on 16S rDNA and genome sequencing, K1 was assigned as <i>Bacillus subtilis</i>. The <i>in vitro</i> results displayed that K1 and its volatile substances could significantly inhibit the mycelia growth of <i>B. cinerea</i>. Grape fruit inoculated with <i>Bacillus</i> K1 showed lower gray mold during treatment. The higher levels of defense-related enzymes, including peroxidase, polyphenol oxidase, and phenylalanine ammonia lyase, were induced in grapes after inoculation. Scanning electron microscopy (SEM) suggested that K1 inhibited mycelial growth <i>via</i> bacterial colonization and antibiosis in grapes. The gas chromatography-mass spectrometry analysis identified 33 volatiles in which dibutyl phthalate was the major compound accounting for 74.28%. Dibutyl phthalate demonstrated strong activity in suppressing the mycelia growth of <i>B. cinerea</i>. Genome bioinformatics analysis revealed that the K1 chromosome harbored many known biosynthesis gene clusters encoding subtilosin, bacillaene, bacillibactin, bacilysin, and fengycin. This study provides a potential biological agent to control diseases of post-harvest grape fruit and improves our understanding of the possible biocontrol mechanisms of the <i>Bacillus</i> strain.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2026-05-31T23:06:25.766Z","creation":"2025-02-19T05:06:02.167Z"},"accession":"S-EPMC9355035","cross_references":{"pubmed":["35935203"],"doi":["10.3389/fmicb.2022.935675"]}}