{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10"],"submitter":["Boucard E"],"pubmed_abstract":["In tissue engineering, cell origin is important to ensure outcome quality. However, the impact of the cell type chosen for seeding in a biocompatible matrix has been less investigated. Here, we investigated the capacity of primary and immortalized fibroblasts of distinct origins to degrade a gelatin/alginate/fibrin (GAF)-based biomaterial. We further established that fibrin was targeted by degradative fibroblasts through the secretion of fibrinolytic matrix-metalloproteinases (MMPs) and urokinase, two types of serine protease. Finally, we demonstrated that besides aprotinin, specific targeting of fibrinolytic MMPs and urokinase led to cell-laden GAF stability for at least forty-eight hours. These results support the use of specific strategies to tune fibrin-based biomaterials degradation o"],"journal":["Frontiers in bioengineering and biotechnology"],"pagination":["920929"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9355319"],"repository":["biostudies-literature"],"pubmed_title":["The degradation of gelatin/alginate/fibrin hydrogels is cell type dependent and can be modulated by targeting fibrinolysis."],"pmcid":["PMC9355319"],"pubmed_authors":["Boucard E","Halary F","Mota C","Coulon F","Vidal L","Hascoet JY"],"additional_accession":[]},"is_claimable":false,"name":"The degradation of gelatin/alginate/fibrin hydrogels is cell type dependent and can be modulated by targeting fibrinolysis.","description":"In tissue engineering, cell origin is important to ensure outcome quality. However, the impact of the cell type chosen for seeding in a biocompatible matrix has been less investigated. Here, we investigated the capacity of primary and immortalized fibroblasts of distinct origins to degrade a gelatin/alginate/fibrin (GAF)-based biomaterial. We further established that fibrin was targeted by degradative fibroblasts through the secretion of fibrinolytic matrix-metalloproteinases (MMPs) and urokinase, two types of serine protease. Finally, we demonstrated that besides aprotinin, specific targeting of fibrinolytic MMPs and urokinase led to cell-laden GAF stability for at least forty-eight hours. These results support the use of specific strategies to tune fibrin-based biomaterials degradation o","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2025-04-21T22:35:27.311Z","creation":"2025-02-19T04:54:02.714Z"},"accession":"S-EPMC9355319","cross_references":{"pubmed":["35935486"],"doi":["10.3389/fbioe.2022.920929"]}}