biostudies-literatureHong HJNational Research Foundation of Korea932-945https://www.ebi.ac.uk/biostudies/studies/S-EPMC9355985biostudies-literatureUnknown54(7)Genetic variations in mitoribosomal subunits and mitochondrial transcription factors are related to type 2 diabetes. However, the role of islet mitoribosomes in the development of type 2 diabetes has not been determined. We investigated the effects of the mitoribosomal gene on β-cell function and glucose homeostasis. Mitoribosomal gene expression was analyzed in datasets from the NCBI GEO website (GSE25724, GSE76894, and GSE76895) and the European Nucleotide Archive (ERP017126), which contain the transcriptomes of type 2 diabetic and nondiabetic organ donors. We found deregulation of most mitoribosomal genes in islets from individuals with type 2 diabetes, including partial downregulation of CRIF1. The phenotypes of haploinsufficiency in a single mitoribosomal gene were examined using β-ceExperimental & molecular medicineMitoribosome insufficiency in β cells is associated with type 2 diabetes-like islet failure.PMC9355985NRF-2020R1A2C20109642017R1A5A2015369NRF-2017R1E1A1A01075126Chang JYKang BEPark JHMoon JHKim HChoi HKim HJHong HJKang YEShong MKim JTRyu DPark KSKang SGRo HJLee CHKim YKJoung KHChoi MJLee YJo YJun SfalseMitoribosome insufficiency in β cells is associated with type 2 diabetes-like islet failure.Genetic variations in mitoribosomal subunits and mitochondrial transcription factors are related to type 2 diabetes. However, the role of islet mitoribosomes in the development of type 2 diabetes has not been determined. We investigated the effects of the mitoribosomal gene on β-cell function and glucose homeostasis. Mitoribosomal gene expression was analyzed in datasets from the NCBI GEO website (GSE25724, GSE76894, and GSE76895) and the European Nucleotide Archive (ERP017126), which contain the transcriptomes of type 2 diabetic and nondiabetic organ donors. We found deregulation of most mitoribosomal genes in islets from individuals with type 2 diabetes, including partial downregulation of CRIF1. The phenotypes of haploinsufficiency in a single mitoribosomal gene were examined using β-ce2022-01-01T00:00:00Z2022 Jul2026-05-09T15:38:41.883Z2024-11-06T07:21:20.386ZS-EPMC93559853580419010.1038/s12276-022-00797-x