{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["Yap JMG"],"pubmed_abstract":["Fibroblasts of different origins are known to possess stromal memory after inflammatory episodes. However, there are no studies exploring human lung fibroblast memory which may predict a subsequent inflammatory response in chronic respiratory diseases and COVID-19. MRC-5 and HF19 human lung fibroblast cell lines were treated using different primary and secondary stimulus combinations: TNFα-WD-TNFα, Poly (I:C)-WD-TNFα, TNFα-WD-Poly (I:C), or LPS-WD-TNFα with a 24-h rest period (withdrawal period; WD) between the two 24-h stimulations. TLR3 and NF-κB inhibitors were used to determine pathways involved. The effect of SARS-Cov-2 spike protein to inflammatory response of lung fibroblasts was also investigated. mRNA expressions of genes and IL6 release were measured using qRT-PCR and ELISA, resp"],"journal":["Frontiers in immunology"],"pagination":["921728"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9356221"],"repository":["biostudies-literature"],"pubmed_title":["Human Lung Fibroblasts Exhibit Induced Inflammation Memory <i>via</i> Increased <i>IL6</i> Gene Expression and Release."],"pmcid":["PMC9356221"],"pubmed_authors":["Takeda N","Ohkubo H","Uemura T","Yap JMG","Fukumitsu K","Ito Y","Niimi A","Fukuda S","Tajiri T","Ueda T","Ugawa S","Maeno K","Kanemitsu Y","Oguri T"],"additional_accession":[]},"is_claimable":false,"name":"Human Lung Fibroblasts Exhibit Induced Inflammation Memory <i>via</i> Increased <i>IL6</i> Gene Expression and Release.","description":"Fibroblasts of different origins are known to possess stromal memory after inflammatory episodes. However, there are no studies exploring human lung fibroblast memory which may predict a subsequent inflammatory response in chronic respiratory diseases and COVID-19. MRC-5 and HF19 human lung fibroblast cell lines were treated using different primary and secondary stimulus combinations: TNFα-WD-TNFα, Poly (I:C)-WD-TNFα, TNFα-WD-Poly (I:C), or LPS-WD-TNFα with a 24-h rest period (withdrawal period; WD) between the two 24-h stimulations. TLR3 and NF-κB inhibitors were used to determine pathways involved. The effect of SARS-Cov-2 spike protein to inflammatory response of lung fibroblasts was also investigated. mRNA expressions of genes and IL6 release were measured using qRT-PCR and ELISA, resp","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2025-05-29T20:50:02.158Z","creation":"2024-11-07T10:58:21.889Z"},"accession":"S-EPMC9356221","cross_references":{"pubmed":["35941890"],"doi":["10.3389/fimmu.2022.921728"]}}