biostudies-literatureUnknown13(8)Liu RIn view of the important roles played by Kinetochore proteins in mitosis, we believed that they may contribute to the development and progression of human cancers, which has been reported recently elsewhere. Kinetochore-associated 1 (KNTC1) participates in the segregation of sister chromatids during mitosis, the effects of which on non-small-cell lung cancer (NSCLC) remain unclear. Here, we sought to identify the biological significance of KNTC1 in NSCLC. KNTC1 protein expression in NSCLC tissues was investigated by immunohistochemistry. Lentivirus delivered short hairpin RNA (shRNA) was utilized to establish KNTC1 silence NSCLC cell lines. The effects of KNTC1 depletion on NSCLC cell proliferation, migration, apoptosis, and tumor formation were analyzed by MTT assay, wound-healing assay, Cell death & disease685https://www.ebi.ac.uk/biostudies/studies/S-EPMC9357013biostudies-literatureshRNA‑mediated knockdown of KNTC1 inhibits non-small-cell lung cancer through regulating PSMB8.PMC9357013Luo QGuo ZHuang JRen JLiu RTan QfalseshRNA‑mediated knockdown of KNTC1 inhibits non-small-cell lung cancer through regulating PSMB8.In view of the important roles played by Kinetochore proteins in mitosis, we believed that they may contribute to the development and progression of human cancers, which has been reported recently elsewhere. Kinetochore-associated 1 (KNTC1) participates in the segregation of sister chromatids during mitosis, the effects of which on non-small-cell lung cancer (NSCLC) remain unclear. Here, we sought to identify the biological significance of KNTC1 in NSCLC. KNTC1 protein expression in NSCLC tissues was investigated by immunohistochemistry. Lentivirus delivered short hairpin RNA (shRNA) was utilized to establish KNTC1 silence NSCLC cell lines. The effects of KNTC1 depletion on NSCLC cell proliferation, migration, apoptosis, and tumor formation were analyzed by MTT assay, wound-healing assay, 2022-01-01T00:00:00Z2022 Aug2025-04-18T15:24:33.388Z2025-02-19T02:26:56.933ZS-EPMC93570133593340510.1038/s41419-022-05140-w