{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10"],"submitter":["Chen R"],"pubmed_abstract":["<h4>Purpose</h4>To investigate the expression and possible role of Sirtuin1 or Silent mating-type information regulation 2 homolog-1 (SIRT1) in post-necrotizing enterocolitis stricture.<h4>Materials and methods</h4>The expression characteristics of SIRT1 and TGF-β1 in post-necrotizing enterocolitis stricture were detected by immunohistochemistry. The siRNA-SIRT1 was used to inhibit the expression of SIRT1 in intestinal epithelial cells-6 (IEC-6), and qRT-PCR, WB, and ELISA were utilized to detect the changes of Transforming growth factor-β1 (TGF-β1), nuclear factor (NF)-κB, tumor necrosis factor-α (TNF-α), tight junction protein-1 (ZO-1), and vascular endothelial growth factor (VEGF) expressions. The IEC-6 cell proliferation and migration ability were tested <i>via</i> CCK8 kit and Transwe"],"journal":["Frontiers in pediatrics"],"pagination":["836128"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9357903"],"repository":["biostudies-literature"],"pubmed_title":["Expression and Possible Role of Silent Mating Type Information Regulation 2 Homolog 1 in Post-necrotizing Enterocolitis Stricture <i>in vivo</i> and <i>in vitro</i>."],"pmcid":["PMC9357903"],"pubmed_authors":["Shi B","Gu W","Tou J","Lv C","Zhang L","Chen R","Zhao Y"],"additional_accession":[]},"is_claimable":false,"name":"Expression and Possible Role of Silent Mating Type Information Regulation 2 Homolog 1 in Post-necrotizing Enterocolitis Stricture <i>in vivo</i> and <i>in vitro</i>.","description":"<h4>Purpose</h4>To investigate the expression and possible role of Sirtuin1 or Silent mating-type information regulation 2 homolog-1 (SIRT1) in post-necrotizing enterocolitis stricture.<h4>Materials and methods</h4>The expression characteristics of SIRT1 and TGF-β1 in post-necrotizing enterocolitis stricture were detected by immunohistochemistry. The siRNA-SIRT1 was used to inhibit the expression of SIRT1 in intestinal epithelial cells-6 (IEC-6), and qRT-PCR, WB, and ELISA were utilized to detect the changes of Transforming growth factor-β1 (TGF-β1), nuclear factor (NF)-κB, tumor necrosis factor-α (TNF-α), tight junction protein-1 (ZO-1), and vascular endothelial growth factor (VEGF) expressions. The IEC-6 cell proliferation and migration ability were tested <i>via</i> CCK8 kit and Transwe","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2025-04-05T08:56:33.253Z","creation":"2025-04-05T08:56:33.253Z"},"accession":"S-EPMC9357903","cross_references":{"pubmed":["35958178"],"doi":["10.3389/fped.2022.836128"]}}