biostudies-literatureUnknown10Chen R<h4>Purpose</h4>To investigate the expression and possible role of Sirtuin1 or Silent mating-type information regulation 2 homolog-1 (SIRT1) in post-necrotizing enterocolitis stricture.<h4>Materials and methods</h4>The expression characteristics of SIRT1 and TGF-β1 in post-necrotizing enterocolitis stricture were detected by immunohistochemistry. The siRNA-SIRT1 was used to inhibit the expression of SIRT1 in intestinal epithelial cells-6 (IEC-6), and qRT-PCR, WB, and ELISA were utilized to detect the changes of Transforming growth factor-β1 (TGF-β1), nuclear factor (NF)-κB, tumor necrosis factor-α (TNF-α), tight junction protein-1 (ZO-1), and vascular endothelial growth factor (VEGF) expressions. The IEC-6 cell proliferation and migration ability were tested <i>via</i> CCK8 kit and TransweFrontiers in pediatrics836128https://www.ebi.ac.uk/biostudies/studies/S-EPMC9357903biostudies-literatureExpression and Possible Role of Silent Mating Type Information Regulation 2 Homolog 1 in Post-necrotizing Enterocolitis Stricture <i>in vivo</i> and <i>in vitro</i>.PMC9357903Shi BGu WTou JLv CZhang LChen RZhao YfalseExpression and Possible Role of Silent Mating Type Information Regulation 2 Homolog 1 in Post-necrotizing Enterocolitis Stricture <i>in vivo</i> and <i>in vitro</i>.<h4>Purpose</h4>To investigate the expression and possible role of Sirtuin1 or Silent mating-type information regulation 2 homolog-1 (SIRT1) in post-necrotizing enterocolitis stricture.<h4>Materials and methods</h4>The expression characteristics of SIRT1 and TGF-β1 in post-necrotizing enterocolitis stricture were detected by immunohistochemistry. The siRNA-SIRT1 was used to inhibit the expression of SIRT1 in intestinal epithelial cells-6 (IEC-6), and qRT-PCR, WB, and ELISA were utilized to detect the changes of Transforming growth factor-β1 (TGF-β1), nuclear factor (NF)-κB, tumor necrosis factor-α (TNF-α), tight junction protein-1 (ZO-1), and vascular endothelial growth factor (VEGF) expressions. The IEC-6 cell proliferation and migration ability were tested <i>via</i> CCK8 kit and Transwe2022-01-01T00:00:00Z20222025-04-05T08:56:33.253Z2025-04-05T08:56:33.253ZS-EPMC93579033595817810.3389/fped.2022.836128