biostudies-literatureUnknown60(1)Yang YFNational Natural Science Foundation of China<h4>Context</h4>Yi-Qi Cong-Ming (YQCM) decoction has been widely used to prevent age-related hearing loss (ARHL), the most prevalent neurodegenerative disease in the elderly.<h4>Objective</h4>To explore the mechanism of YQCM decoction in the treatment of ARHL.<h4>Materials and methods</h4>The chemical constituents of YQCM were screened from the Traditional Chinese Medicine Systems Pharmacology Database. Potential targets of YQCM against ARHL were predicted by DrugBank, GeneCards, and OMIM database. Protein-protein network and enrichment analysis were used for exploring possible molecular mechanisms. Molecular docking and an <i>in vitro</i> model of ARHL by exposing auditory cells with 100 μM H₂O₂ for 3 h were applied. Cell viability and mitochondrial membrane potentiaPharmaceutical biology1478-1490https://www.ebi.ac.uk/biostudies/studies/S-EPMC9359200biostudies-literatureNetwork pharmacology and experimental evidence reveal the protective mechanism of Yi-Qi Cong-Ming decoction on age-related hearing loss.PMC9359200Yang YFDong YWu RXShi JRLiu QYan XRChu MFu SPLi NfalseNetwork pharmacology and experimental evidence reveal the protective mechanism of Yi-Qi Cong-Ming decoction on age-related hearing loss.<h4>Context</h4>Yi-Qi Cong-Ming (YQCM) decoction has been widely used to prevent age-related hearing loss (ARHL), the most prevalent neurodegenerative disease in the elderly.<h4>Objective</h4>To explore the mechanism of YQCM decoction in the treatment of ARHL.<h4>Materials and methods</h4>The chemical constituents of YQCM were screened from the Traditional Chinese Medicine Systems Pharmacology Database. Potential targets of YQCM against ARHL were predicted by DrugBank, GeneCards, and OMIM database. Protein-protein network and enrichment analysis were used for exploring possible molecular mechanisms. Molecular docking and an <i>in vitro</i> model of ARHL by exposing auditory cells with 100 μM H₂O₂ for 3 h were applied. Cell viability and mitochondrial membrane potentia2022-01-01T00:00:00Z2022 Dec2025-04-19T12:10:45.184Z2025-04-19T12:10:45.184ZS-EPMC93592003593850410.1080/13880209.2022.2101671