{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["17(10)"],"submitter":["Cai X"],"pubmed_abstract":["High-throughput lysis and proteolytic digestion of biopsy-level tissue specimens is a major bottleneck for clinical proteomics. Here we describe a detailed protocol of pressure cycling technology (PCT)-assisted sample preparation for proteomic analysis of biopsy tissues. A piece of fresh frozen or formalin-fixed paraffin-embedded tissue weighing ~0.1-2 mg is placed in a 150 μL pressure-resistant tube called a PCT-MicroTube with proper lysis buffer. After closing with a PCT-MicroPestle, a batch of 16 PCT-MicroTubes are placed in a Barocycler, which imposes oscillating pressure to the samples from one atmosphere to up to ~3,000 times atmospheric pressure. The pressure cycling schemes are optimized for tissue lysis and protein digestion, and can be programmed in the Barocycler to allow reprod"],"journal":["Nature protocols"],"pagination":["2307-2325"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9362583"],"repository":["biostudies-literature"],"pubmed_title":["High-throughput proteomic sample preparation using pressure cycling technology."],"pmcid":["PMC9362583"],"pubmed_authors":["Xu L","Yi X","Zhu Y","Wu C","Yue L","Guo T","Gao H","Xue Z","Chen C","Yu J","Liu W","Ge W","Sun R","Qian L","Cai X"],"additional_accession":[]},"is_claimable":false,"name":"High-throughput proteomic sample preparation using pressure cycling technology.","description":"High-throughput lysis and proteolytic digestion of biopsy-level tissue specimens is a major bottleneck for clinical proteomics. Here we describe a detailed protocol of pressure cycling technology (PCT)-assisted sample preparation for proteomic analysis of biopsy tissues. A piece of fresh frozen or formalin-fixed paraffin-embedded tissue weighing ~0.1-2 mg is placed in a 150 μL pressure-resistant tube called a PCT-MicroTube with proper lysis buffer. After closing with a PCT-MicroPestle, a batch of 16 PCT-MicroTubes are placed in a Barocycler, which imposes oscillating pressure to the samples from one atmosphere to up to ~3,000 times atmospheric pressure. The pressure cycling schemes are optimized for tissue lysis and protein digestion, and can be programmed in the Barocycler to allow reprod","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2025-04-05T23:57:35.254Z","creation":"2025-04-05T23:57:35.254Z"},"accession":"S-EPMC9362583","cross_references":{"pubmed":["35931778"],"doi":["10.1038/s41596-022-00727-1"]}}