biostudies-literatureUnknown13Correa IAAbbott LaboratoriesConselho Nacional de Desenvolvimento Científico e TecnológicoUniversidade Federal do Rio de JaneiroCoordenação de Aperfeiçoamento de Pessoal de Nível SuperiorInstituto SerrapilheiraFundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de JaneiroSevere acute respiratory syndrome-related coronavirus (SARS-CoV-2) transmission occurs even among fully vaccinated individuals; thus, prompt identification of infected patients is central to control viral circulation. Antigen rapid diagnostic tests (Ag-RDTs) are highly specific, but sensitivity is variable. Discordant RT-qPCR vs. Ag-RDT results are reported, raising the question of whether negative Ag-RDT in positive RT-qPCR samples could imply the absence of infectious viruses. To study the relationship between negative Ag-RDT results with virological, molecular, and serological parameters, we selected a cross-sectional and a follow-up dataset and analyzed virus culture, subgenomic RNA quantification, and sequencing to determine infectious viruses and mutations. We demonstrated that RT-qPCR positive while SARS-CoV-2 Ag-RDT negative discordant results correlate with the absence of infectious virus in nasopharyngeal samples. A decrease in sgRNA detection together with an expected increase in detectable anti-S and anti-N IgGs was also verified in these samples. The data clearly demonstrate that a negative Ag-RDT sample is less likely to harbor infectious SARS-CoV-2 and, consequently, has a lower transmissible potential.Frontiers in microbiology912138https://www.ebi.ac.uk/biostudies/studies/S-EPMC9364907biostudies-literatureA SARS-CoV-2 Negative Antigen Rapid Diagnostic in RT-qPCR Positive Samples Correlates With a Low Likelihood of Infectious Viruses in the Nasopharynx.PMC9364907Vijesurier RCorrea IAda Costa LJPolicarpo CFerreira ODCMariani DCunha MSCloherty GARibeiro LJda Silva GPTanuri AGalliez RMHackett JLeitao ICde Souza MRBerg MMoreira FRRCampos MFFaffe DSGoncalves CCARodgers MAda Cruz TFCGonzales LNascimento ERDSMaia RACastineiras TMPPfalseA SARS-CoV-2 Negative Antigen Rapid Diagnostic in RT-qPCR Positive Samples Correlates With a Low Likelihood of Infectious Viruses in the Nasopharynx.Severe acute respiratory syndrome-related coronavirus (SARS-CoV-2) transmission occurs even among fully vaccinated individuals; thus, prompt identification of infected patients is central to control viral circulation. Antigen rapid diagnostic tests (Ag-RDTs) are highly specific, but sensitivity is variable. Discordant RT-qPCR vs. Ag-RDT results are reported, raising the question of whether negative Ag-RDT in positive RT-qPCR samples could imply the absence of infectious viruses. To study the relationship between negative Ag-RDT results with virological, molecular, and serological parameters, we selected a cross-sectional and a follow-up dataset and analyzed virus culture, subgenomic RNA quantification, and sequencing to determine infectious viruses and mutations. We demonstrated that RT-qPCR positive while SARS-CoV-2 Ag-RDT negative discordant results correlate with the absence of infectious virus in nasopharyngeal samples. A decrease in sgRNA detection together with an expected increase in detectable anti-S and anti-N IgGs was also verified in these samples. The data clearly demonstrate that a negative Ag-RDT sample is less likely to harbor infectious SARS-CoV-2 and, consequently, has a lower transmissible potential.2022-01-01T00:00:00Z20222024-10-18T22:40:15.836Z2024-10-18T22:40:15.836ZS-EPMC93649073596671410.3389/fmicb.2022.912138