biostudies-literatureDylgjeri ECancer Research UKHHS | NIH | National Cancer InstituteNCI NIH HHSProstate Cancer Foundation1446-1459https://www.ebi.ac.uk/biostudies/studies/S-EPMC9365345biostudies-literatureUnknown28(7)<h4>Purpose</h4>DNA-dependent protein kinase catalytic subunit (DNA-PKcs, herein referred as DNA-PK) is a multifunctional kinase of high cancer relevance. DNA-PK is deregulated in multiple tumor types, including prostate cancer, and is associated with poor outcomes. DNA-PK was previously nominated as a therapeutic target and DNA-PK inhibitors are currently undergoing clinical investigation. Although DNA-PK is well studied in DNA repair and transcriptional regulation, much remains to be understood about the way by which DNA-PK drives aggressive disease phenotypes.<h4>Experimental design</h4>Here, unbiased proteomic and metabolomic approaches in clinically relevant tumor models uncovered a novel role of DNA-PK in metabolic regulation of cancer progression. DNA-PK regulation of metabolism wasClinical cancer research : an official journal of the American Association for Cancer ResearchA Novel Role for DNA-PK in Metabolism by Regulating Glycolysis in Castration-Resistant Prostate Cancer.PMC9365345204115R01CA18256905P30 CA056036F99CA2122255R01CA17640105F99 CA212225Lallas CDHolst JMcCue PAVasilevskaya IPang ADylgjeri EChand SButler LMMcNair CMShafi AAKothari VSemenova GSchiewer MJGomella LGKnudsen KESeifert ELKelly WKGuan YFCarroll JSGallagher PTGoodwin JFMandigo ACMcCann JJIrani SfalseA Novel Role for DNA-PK in Metabolism by Regulating Glycolysis in Castration-Resistant Prostate Cancer.<h4>Purpose</h4>DNA-dependent protein kinase catalytic subunit (DNA-PKcs, herein referred as DNA-PK) is a multifunctional kinase of high cancer relevance. DNA-PK is deregulated in multiple tumor types, including prostate cancer, and is associated with poor outcomes. DNA-PK was previously nominated as a therapeutic target and DNA-PK inhibitors are currently undergoing clinical investigation. Although DNA-PK is well studied in DNA repair and transcriptional regulation, much remains to be understood about the way by which DNA-PK drives aggressive disease phenotypes.<h4>Experimental design</h4>Here, unbiased proteomic and metabolomic approaches in clinically relevant tumor models uncovered a novel role of DNA-PK in metabolic regulation of cancer progression. DNA-PK regulation of metabolism was2022-01-01T00:00:00Z2022 Apr2025-04-22T04:27:49.611Z2025-04-05T21:00:08.837ZS-EPMC93653453507886110.1158/1078-0432.CCR-21-1846