{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["39(7)"],"submitter":["Lal A"],"funding":["School of Medicine, University of South Carolina"],"pubmed_abstract":["<h4>Purpose</h4>Successful identification of transcriptomic biomarkers within human IVF embryos may enhance implantation prediction and provide insights not available through conventional embryo biopsy genomic analysis. We demonstrate proof-of-concept for a methodology to assess overall embryo gene expression using qPCR with blastocoel fluid-conditioned media by examining the comparative presence of apoptotic genes.<h4>Methods</h4>Blastocoel fluid-conditioned media were collected from 19 embryos (11 euploid) following trophectoderm biopsy of day-5 ICSI-IVF blastocysts. Media were assessed for apoptotic gene expression via qPCR. Statistical analysis of gene expression was conducted via Wilcoxon Signed-Ranks test (overall expression), multivariate ANOVA (functional gene groups), and chi-squa"],"journal":["Journal of assisted reproduction and genetics"],"pagination":["1515-1522"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9365898"],"repository":["biostudies-literature"],"pubmed_title":["Apoptotic qPCR gene expression array analysis demonstrates proof-of-concept for rapid blastocoel fluid-conditioned media molecular prediction."],"pmcid":["PMC9365898"],"pubmed_authors":["Chosed RJ","Chang TA","Lal A","Zimmerman S","Fowler LA","Wininger JD","Kranyak A","Robinson RD","Roudebush WE","Athavale D","Doran A","Blalock J","Barre A"],"additional_accession":[]},"is_claimable":false,"name":"Apoptotic qPCR gene expression array analysis demonstrates proof-of-concept for rapid blastocoel fluid-conditioned media molecular prediction.","description":"<h4>Purpose</h4>Successful identification of transcriptomic biomarkers within human IVF embryos may enhance implantation prediction and provide insights not available through conventional embryo biopsy genomic analysis. We demonstrate proof-of-concept for a methodology to assess overall embryo gene expression using qPCR with blastocoel fluid-conditioned media by examining the comparative presence of apoptotic genes.<h4>Methods</h4>Blastocoel fluid-conditioned media were collected from 19 embryos (11 euploid) following trophectoderm biopsy of day-5 ICSI-IVF blastocysts. Media were assessed for apoptotic gene expression via qPCR. Statistical analysis of gene expression was conducted via Wilcoxon Signed-Ranks test (overall expression), multivariate ANOVA (functional gene groups), and chi-squa","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jul","modification":"2025-04-26T07:40:46.786Z","creation":"2025-04-06T12:23:27.606Z"},"accession":"S-EPMC9365898","cross_references":{"pubmed":["35543804"],"doi":["10.1007/s10815-022-02510-3"]}}