biostudies-literatureUnknown39(7)Lal ASchool of Medicine, University of South Carolina<h4>Purpose</h4>Successful identification of transcriptomic biomarkers within human IVF embryos may enhance implantation prediction and provide insights not available through conventional embryo biopsy genomic analysis. We demonstrate proof-of-concept for a methodology to assess overall embryo gene expression using qPCR with blastocoel fluid-conditioned media by examining the comparative presence of apoptotic genes.<h4>Methods</h4>Blastocoel fluid-conditioned media were collected from 19 embryos (11 euploid) following trophectoderm biopsy of day-5 ICSI-IVF blastocysts. Media were assessed for apoptotic gene expression via qPCR. Statistical analysis of gene expression was conducted via Wilcoxon Signed-Ranks test (overall expression), multivariate ANOVA (functional gene groups), and chi-squaJournal of assisted reproduction and genetics1515-1522https://www.ebi.ac.uk/biostudies/studies/S-EPMC9365898biostudies-literatureApoptotic qPCR gene expression array analysis demonstrates proof-of-concept for rapid blastocoel fluid-conditioned media molecular prediction.PMC9365898Chosed RJChang TALal AZimmerman SFowler LAWininger JDKranyak ARobinson RDRoudebush WEAthavale DDoran ABlalock JBarre AfalseApoptotic qPCR gene expression array analysis demonstrates proof-of-concept for rapid blastocoel fluid-conditioned media molecular prediction.<h4>Purpose</h4>Successful identification of transcriptomic biomarkers within human IVF embryos may enhance implantation prediction and provide insights not available through conventional embryo biopsy genomic analysis. We demonstrate proof-of-concept for a methodology to assess overall embryo gene expression using qPCR with blastocoel fluid-conditioned media by examining the comparative presence of apoptotic genes.<h4>Methods</h4>Blastocoel fluid-conditioned media were collected from 19 embryos (11 euploid) following trophectoderm biopsy of day-5 ICSI-IVF blastocysts. Media were assessed for apoptotic gene expression via qPCR. Statistical analysis of gene expression was conducted via Wilcoxon Signed-Ranks test (overall expression), multivariate ANOVA (functional gene groups), and chi-squa2022-01-01T00:00:00Z2022 Jul2025-04-26T07:40:46.786Z2025-04-06T12:23:27.606ZS-EPMC93658983554380410.1007/s10815-022-02510-3