biostudies-literatureHering AEuropean Research CouncilAustralian Research Council9100-9103https://www.ebi.ac.uk/biostudies/studies/S-EPMC9367247biostudies-literatureUnknown58(65)The oxime ligation is a valuable bioorthogonal conjugation reaction but with limited compatibility with disulfide-rich peptides/proteins and time-sensitive applications. Here we overcome these limitations by introducing a strategy that supports regiospecific control, oxidative folding, production of stable aminooxy-precursors for on-demand modification, and complete ligation within 5 min.Chemical communications (Cambridge, England)Expanding the versatility and scope of the oxime ligation: rapid bioconjugation to disulfide-rich peptides.PMC9367247DE150100784, DP190101667, FT210100266714366Hering AMuttenthaler MBraga Emidio NfalseExpanding the versatility and scope of the oxime ligation: rapid bioconjugation to disulfide-rich peptides.The oxime ligation is a valuable bioorthogonal conjugation reaction but with limited compatibility with disulfide-rich peptides/proteins and time-sensitive applications. Here we overcome these limitations by introducing a strategy that supports regiospecific control, oxidative folding, production of stable aminooxy-precursors for on-demand modification, and complete ligation within 5 min.2022-01-01T00:00:00Z2022 Aug2025-04-18T18:07:26.22Z2025-04-07T05:42:06.371ZS-EPMC93672473588048210.1039/d2cc03752a