biostudies-literatureMena MAsociación Española Contra el CáncerInstituto de Salud Carlos IIIAgency for Administration of University and Research3787https://www.ebi.ac.uk/biostudies/studies/S-EPMC9367257biostudies-literatureUnknown14(15)<b>Background</b>: Tests or test algorithms for diagnosing HPV-driven oral cavity and laryngeal head and neck carcinomas (HNC) have not been yet validated, and the differences among oral cavity and laryngeal sites have not been comprehensively evaluated. We aimed to assess the utility of a diagnostic algorithm for the detection of HPV-driven oral cavity (OCC), oropharyngeal (OPC) and laryngeal (LC) carcinomas using HPV-DNA testing followed by p16^INK4a immunohistochemistry, taking E6*I mRNA detection as the reference standard. <b>Methods:</b> Formalin-fixed paraffin-embedded OCC, OPC, and LC carcinomas were collected from pathology archives in 29 countries. All samples were subjected to histopathological evaluation, DNA quality control, and HPV-DNA detection. All HPV-DNA-positiveCancersConcordance of p16^INK4a and E6*I mRNA among HPV-DNA-Positive Oropharyngeal, Laryngeal, and Oral Cavity Carcinomas from the ICO International Study.PMC93672572017SGR1085FIS PI081535, FIS PI1102096, FIS PI1102104, and CIBERESP CB06/02/007Lloveras Rubio BMehanna HTous SBosch FXOn Behalf Of The Ico International Hpv In Head And Neck Cancer Study GroupAlos LQuiros BAlejo MDe Sanjose STommasino MLeon Vintro XMorey FPavon MAClavero OMunoz NQuint WAlemany LWang XTaberna MPawlita MMena MfalseConcordance of p16^INK4a and E6*I mRNA among HPV-DNA-Positive Oropharyngeal, Laryngeal, and Oral Cavity Carcinomas from the ICO International Study.<b>Background</b>: Tests or test algorithms for diagnosing HPV-driven oral cavity and laryngeal head and neck carcinomas (HNC) have not been yet validated, and the differences among oral cavity and laryngeal sites have not been comprehensively evaluated. We aimed to assess the utility of a diagnostic algorithm for the detection of HPV-driven oral cavity (OCC), oropharyngeal (OPC) and laryngeal (LC) carcinomas using HPV-DNA testing followed by p16^INK4a immunohistochemistry, taking E6*I mRNA detection as the reference standard. <b>Methods:</b> Formalin-fixed paraffin-embedded OCC, OPC, and LC carcinomas were collected from pathology archives in 29 countries. All samples were subjected to histopathological evaluation, DNA quality control, and HPV-DNA detection. All HPV-DNA-positive2022-01-01T00:00:00Z2022 Aug2025-04-18T18:09:12.894Z2025-02-19T03:32:20.164ZS-EPMC93672573595445110.3390/cancers14153787