{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yao Q"],"funding":["The National Key Research and Development Program of China","The Open Funds of the State Key Laboratory of Veterinary Aetiological Biology, Lanzhou Vet-erinary Research Institute, Chinese Academy of Agricultural Sciences","The National Natural Science Foundation of China"],"pagination":["1953"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9367258"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(15)"],"pubmed_abstract":["<i>C. parvum</i> is an important diarrheal pathogen in humans and animals, especially in young hosts. To accurately and rapidly detect <i>C. parvum</i> infection in calves, we established a nano-PCR assay targeting the <i>cgd3_330</i> gene for the specific detection of <i>C. parvum</i>. This nano-PCR assay was ten times more sensitive than that of the normal PCR assay by applying the same primers and did not cross-react with <i>C. andersoni</i>, <i>C. bovis</i>, <i>C. ryanae</i>, <i>Balantidium coli</i>, <i>Enterocytozoon bieneusi</i>, <i>Giardia lamblia</i>, and <i>Blastocystis</i> sp. To further test the nano-PCR in clinical settings, a total of 20 faecal samples from calves were examined by using the nano-PCR, the normal PCR, and the nested PCR assays. The positive rates were 30% (6/20), 30% (6/20), and 25% (5/20) for the nano-PCR, the normal PCR, and the nested PCR assays, respectively, indicating that the nano-PCR and the normal PCR assays had the same positive rate (30%). Taken together, the present study could provide a candidate method for the specific detection of <i>C. parvum</i> infection in calves in clinical settings."],"journal":["Animals : an open access journal from MDPI"],"pubmed_title":["Development and Preliminary Evaluation of a Nanoparticle-Assisted PCR Assay for the Detection of <i>Cryptosporidium parvum</i> in Calves."],"pmcid":["PMC9367258"],"funding_grant_id":["SKLVEB2020KFKT015","2017YFD0501305","32072890"],"pubmed_authors":["Yang X","Huang S","Zhao G","Yao Q","Wang Y","Wang J","Song J"],"additional_accession":[]},"is_claimable":false,"name":"Development and Preliminary Evaluation of a Nanoparticle-Assisted PCR Assay for the Detection of <i>Cryptosporidium parvum</i> in Calves.","description":"<i>C. parvum</i> is an important diarrheal pathogen in humans and animals, especially in young hosts. To accurately and rapidly detect <i>C. parvum</i> infection in calves, we established a nano-PCR assay targeting the <i>cgd3_330</i> gene for the specific detection of <i>C. parvum</i>. This nano-PCR assay was ten times more sensitive than that of the normal PCR assay by applying the same primers and did not cross-react with <i>C. andersoni</i>, <i>C. bovis</i>, <i>C. ryanae</i>, <i>Balantidium coli</i>, <i>Enterocytozoon bieneusi</i>, <i>Giardia lamblia</i>, and <i>Blastocystis</i> sp. To further test the nano-PCR in clinical settings, a total of 20 faecal samples from calves were examined by using the nano-PCR, the normal PCR, and the nested PCR assays. The positive rates were 30% (6/20), 30% (6/20), and 25% (5/20) for the nano-PCR, the normal PCR, and the nested PCR assays, respectively, indicating that the nano-PCR and the normal PCR assays had the same positive rate (30%). Taken together, the present study could provide a candidate method for the specific detection of <i>C. parvum</i> infection in calves in clinical settings.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Aug","modification":"2025-04-05T08:56:23.861Z","creation":"2025-02-19T03:32:22.666Z"},"accession":"S-EPMC9367258","cross_references":{"pubmed":["35953942"],"doi":["10.3390/ani12151953"]}}