biostudies-literatureYao QThe National Key Research and Development Program of ChinaThe Open Funds of the State Key Laboratory of Veterinary Aetiological Biology, Lanzhou Vet-erinary Research Institute, Chinese Academy of Agricultural SciencesThe National Natural Science Foundation of China1953https://www.ebi.ac.uk/biostudies/studies/S-EPMC9367258biostudies-literatureUnknown12(15)<i>C. parvum</i> is an important diarrheal pathogen in humans and animals, especially in young hosts. To accurately and rapidly detect <i>C. parvum</i> infection in calves, we established a nano-PCR assay targeting the <i>cgd3_330</i> gene for the specific detection of <i>C. parvum</i>. This nano-PCR assay was ten times more sensitive than that of the normal PCR assay by applying the same primers and did not cross-react with <i>C. andersoni</i>, <i>C. bovis</i>, <i>C. ryanae</i>, <i>Balantidium coli</i>, <i>Enterocytozoon bieneusi</i>, <i>Giardia lamblia</i>, and <i>Blastocystis</i> sp. To further test the nano-PCR in clinical settings, a total of 20 faecal samples from calves were examined by using the nano-PCR, the normal PCR, and the nested PCR assays. The positive rates were 30% (6/20), 30% (6/20), and 25% (5/20) for the nano-PCR, the normal PCR, and the nested PCR assays, respectively, indicating that the nano-PCR and the normal PCR assays had the same positive rate (30%). Taken together, the present study could provide a candidate method for the specific detection of <i>C. parvum</i> infection in calves in clinical settings.Animals : an open access journal from MDPIDevelopment and Preliminary Evaluation of a Nanoparticle-Assisted PCR Assay for the Detection of <i>Cryptosporidium parvum</i> in Calves.PMC9367258SKLVEB2020KFKT0152017YFD050130532072890Yang XHuang SZhao GYao QWang YWang JSong JfalseDevelopment and Preliminary Evaluation of a Nanoparticle-Assisted PCR Assay for the Detection of <i>Cryptosporidium parvum</i> in Calves.<i>C. parvum</i> is an important diarrheal pathogen in humans and animals, especially in young hosts. To accurately and rapidly detect <i>C. parvum</i> infection in calves, we established a nano-PCR assay targeting the <i>cgd3_330</i> gene for the specific detection of <i>C. parvum</i>. This nano-PCR assay was ten times more sensitive than that of the normal PCR assay by applying the same primers and did not cross-react with <i>C. andersoni</i>, <i>C. bovis</i>, <i>C. ryanae</i>, <i>Balantidium coli</i>, <i>Enterocytozoon bieneusi</i>, <i>Giardia lamblia</i>, and <i>Blastocystis</i> sp. To further test the nano-PCR in clinical settings, a total of 20 faecal samples from calves were examined by using the nano-PCR, the normal PCR, and the nested PCR assays. The positive rates were 30% (6/20), 30% (6/20), and 25% (5/20) for the nano-PCR, the normal PCR, and the nested PCR assays, respectively, indicating that the nano-PCR and the normal PCR assays had the same positive rate (30%). Taken together, the present study could provide a candidate method for the specific detection of <i>C. parvum</i> infection in calves in clinical settings.2022-01-01T00:00:00Z2022 Aug2025-04-05T08:56:23.861Z2025-02-19T03:32:22.666ZS-EPMC93672583595394210.3390/ani12151953