biostudies-literatureMadorran ESlovenian Research AgencyMinistry of Education, Science and Sport2314https://www.ebi.ac.uk/biostudies/studies/S-EPMC9367465biostudies-literatureUnknown11(15)Determining the viability of cells is fraught with many uncertainties. It is often difficult to determine whether a cell is still alive, approaching the point of no return, or dead. Today, there are many methods for determining cell viability. Most rely on an indirect determination of cell death (metabolism, molecular transport, and leakage, to name a few). In contrast, we have developed a promising novel method for a "direct" determination of cell viability. The potential method assesses cell membrane integrity (which is essential for all viable cells) by measuring the electrical potential of the cell membrane. To test the assay, we chose two different cell types, blood macrophages (TLT) and breast cancer epithelial cells (MCF 7). We exposed them to seven different toxic scenarios (arseniCellsA Promising Method for the Determination of Cell Viability: The Membrane Potential Cell Viability Assay.PMC9367465P3-0036, J3-9272, L4-1843, and P3-0396C3330-19-952029Maver UArsov ZMadorran ERozanc JStozer AfalseA Promising Method for the Determination of Cell Viability: The Membrane Potential Cell Viability Assay.Determining the viability of cells is fraught with many uncertainties. It is often difficult to determine whether a cell is still alive, approaching the point of no return, or dead. Today, there are many methods for determining cell viability. Most rely on an indirect determination of cell death (metabolism, molecular transport, and leakage, to name a few). In contrast, we have developed a promising novel method for a "direct" determination of cell viability. The potential method assesses cell membrane integrity (which is essential for all viable cells) by measuring the electrical potential of the cell membrane. To test the assay, we chose two different cell types, blood macrophages (TLT) and breast cancer epithelial cells (MCF 7). We exposed them to seven different toxic scenarios (arseni2022-01-01T00:00:00Z2022 Jul2025-04-19T22:58:28.028Z2025-02-19T03:32:04.868ZS-EPMC93674653595415910.3390/cells11152314