biostudies-literatureLiu AShenzhen Second People’s Hospital487-500https://www.ebi.ac.uk/biostudies/studies/S-EPMC9367658biostudies-literatureUnknown11(1)White adipose tissue wasting plays a critical role in the development and progression of cancer cachexia. However, the mechanism behind the loss of adipose tissue remains ill-defined. In this study, we found that cancer cell-derived exosomes highly expressed miR-425-3p. Administration of cancer cell-derived exosomes significantly inhibited proliferation and differentiation of human preadipocytes-viscereal (HPA-v) cells. In mature adipocytes, cancer cell-derived exosomes activated cAMP/PKA signalling and lipophagy, leading to adipocyte lipolysis and browning of white adipocytes. These exosomes-induced alterations were almost abolished by endocytosis inhibitor cytochalasin D (CytoD) and antagomiR-425-3p, or reproduced by miR-425-3p mimics. In addition, bioinformatics analysis and luciferase AdipocyteCancer cell-derived exosomal miR-425-3p induces white adipocyte atrophy.PMC93676582019060014Pan WZhuang SZhang HLiu ATang YfalseCancer cell-derived exosomal miR-425-3p induces white adipocyte atrophy.White adipose tissue wasting plays a critical role in the development and progression of cancer cachexia. However, the mechanism behind the loss of adipose tissue remains ill-defined. In this study, we found that cancer cell-derived exosomes highly expressed miR-425-3p. Administration of cancer cell-derived exosomes significantly inhibited proliferation and differentiation of human preadipocytes-viscereal (HPA-v) cells. In mature adipocytes, cancer cell-derived exosomes activated cAMP/PKA signalling and lipophagy, leading to adipocyte lipolysis and browning of white adipocytes. These exosomes-induced alterations were almost abolished by endocytosis inhibitor cytochalasin D (CytoD) and antagomiR-425-3p, or reproduced by miR-425-3p mimics. In addition, bioinformatics analysis and luciferase 2022-01-01T00:00:00Z2022 Dec2025-04-18T18:08:53.534Z2025-04-07T05:43:13.405ZS-EPMC93676583594183310.1080/21623945.2022.2108558