{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11(15)"],"submitter":["Hollenhorst MI"],"pubmed_abstract":["Mucociliary clearance is a primary defence mechanism of the airways consisting of two components, ciliary beating and transepithelial ion transport (I<sub>SC</sub>). Specialised chemosensory cholinergic epithelial cells, named brush cells (BC), are involved in regulating various physiological and immunological processes. However, it remains unclear if BC influence I<sub>SC</sub>. In murine tracheae, denatonium, a taste receptor agonist, reduced basal I<sub>SC</sub> in a concentration-dependent manner (EC<sub>50</sub> 397 µM). The inhibition of bitter taste signalling components with gallein (G<sub>βγ</sub> subunits), U73122 (phospholipase C), 2-APB (IP3-receptors) or with TPPO (Trpm5, transient receptor potential-melastatin 5 channel) reduced the denatonium effect. Supportively, the I<sub>"],"journal":["Cells"],"pagination":["2411"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9367940"],"repository":["biostudies-literature"],"pubmed_title":["Taste Receptor Activation in Tracheal Brush Cells by Denatonium Modulates ENaC Channels via Ca<sup>2+</sup>, cAMP and ACh."],"pmcid":["PMC9367940"],"pubmed_authors":["Maxeiner S","Chubanov V","Boehm U","Elhawy MI","Krasteva-Christ G","Gudermann T","Flockerzi V","Kumar P","Salah A","Zimmer M","Hollenhorst MI","Evers SB"],"additional_accession":[]},"is_claimable":false,"name":"Taste Receptor Activation in Tracheal Brush Cells by Denatonium Modulates ENaC Channels via Ca<sup>2+</sup>, cAMP and ACh.","description":"Mucociliary clearance is a primary defence mechanism of the airways consisting of two components, ciliary beating and transepithelial ion transport (I<sub>SC</sub>). Specialised chemosensory cholinergic epithelial cells, named brush cells (BC), are involved in regulating various physiological and immunological processes. However, it remains unclear if BC influence I<sub>SC</sub>. In murine tracheae, denatonium, a taste receptor agonist, reduced basal I<sub>SC</sub> in a concentration-dependent manner (EC<sub>50</sub> 397 µM). The inhibition of bitter taste signalling components with gallein (G<sub>βγ</sub> subunits), U73122 (phospholipase C), 2-APB (IP3-receptors) or with TPPO (Trpm5, transient receptor potential-melastatin 5 channel) reduced the denatonium effect. Supportively, the I<sub>","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Aug","modification":"2025-04-05T23:57:55.919Z","creation":"2025-02-19T03:31:10.05Z"},"accession":"S-EPMC9367940","cross_references":{"pubmed":["35954259"],"doi":["10.3390/cells11152411"]}}