biostudies-literatureUnknown11(15)Hollenhorst MIMucociliary clearance is a primary defence mechanism of the airways consisting of two components, ciliary beating and transepithelial ion transport (I_SC). Specialised chemosensory cholinergic epithelial cells, named brush cells (BC), are involved in regulating various physiological and immunological processes. However, it remains unclear if BC influence I_SC. In murine tracheae, denatonium, a taste receptor agonist, reduced basal I_SC in a concentration-dependent manner (EC₅₀ 397 µM). The inhibition of bitter taste signalling components with gallein (G_βγ subunits), U73122 (phospholipase C), 2-APB (IP3-receptors) or with TPPO (Trpm5, transient receptor potential-melastatin 5 channel) reduced the denatonium effect. Supportively, the I<sub>Cells2411https://www.ebi.ac.uk/biostudies/studies/S-EPMC9367940biostudies-literatureTaste Receptor Activation in Tracheal Brush Cells by Denatonium Modulates ENaC Channels via Ca²⁺, cAMP and ACh.PMC9367940Maxeiner SChubanov VBoehm UElhawy MIKrasteva-Christ GGudermann TFlockerzi VKumar PSalah AZimmer MHollenhorst MIEvers SBfalseTaste Receptor Activation in Tracheal Brush Cells by Denatonium Modulates ENaC Channels via Ca²⁺, cAMP and ACh.Mucociliary clearance is a primary defence mechanism of the airways consisting of two components, ciliary beating and transepithelial ion transport (I_SC). Specialised chemosensory cholinergic epithelial cells, named brush cells (BC), are involved in regulating various physiological and immunological processes. However, it remains unclear if BC influence I_SC. In murine tracheae, denatonium, a taste receptor agonist, reduced basal I_SC in a concentration-dependent manner (EC₅₀ 397 µM). The inhibition of bitter taste signalling components with gallein (G_βγ subunits), U73122 (phospholipase C), 2-APB (IP3-receptors) or with TPPO (Trpm5, transient receptor potential-melastatin 5 channel) reduced the denatonium effect. Supportively, the I<sub>2022-01-01T00:00:00Z2022 Aug2025-04-05T23:57:55.919Z2025-02-19T03:31:10.05ZS-EPMC93679403595425910.3390/cells11152411