biostudies-literatureKawase AJapan Society for the Promotion of Science8573https://www.ebi.ac.uk/biostudies/studies/S-EPMC9369313biostudies-literatureUnknown23(15)SLC25A39/40, involved in mitochondrial GSH (mGSH) import from the cytoplasm, is essential for protection against oxidative stress and mitochondrial dysfunction. We examined the effects of cholestasis, through bile duct ligation (BDL) and lipopolysaccharide (LPS)-induced inflammation in mice, on Slc25a39/40 expression. Additionally, we used human clear cell renal carcinoma (KMRC-1) cells to elucidate the mechanism of regulation of SLC25A39/40 expression in the kidneys after LPS treatment. BDL resulted in a decrease in <i>Slc25a39</i> mRNA in the liver and a decrease in <i>Slc25a39/40</i> mRNA and protein in the kidneys. Consequently, there was a significant decrease in mGSH levels in the kidneys of BDL mice compared with those in sham mice. LPS treatment resulted in increased <i>Slc25a40</iInternational journal of molecular sciencesSlc25a39 and Slc25a40 Expression in Mice with Bile Duct Ligation or Lipopolysaccharide Treatment.PMC936931321K06698Kawase AShimada HHatanaka MMatsuda NIwaki MfalseSlc25a39 and Slc25a40 Expression in Mice with Bile Duct Ligation or Lipopolysaccharide Treatment.SLC25A39/40, involved in mitochondrial GSH (mGSH) import from the cytoplasm, is essential for protection against oxidative stress and mitochondrial dysfunction. We examined the effects of cholestasis, through bile duct ligation (BDL) and lipopolysaccharide (LPS)-induced inflammation in mice, on Slc25a39/40 expression. Additionally, we used human clear cell renal carcinoma (KMRC-1) cells to elucidate the mechanism of regulation of SLC25A39/40 expression in the kidneys after LPS treatment. BDL resulted in a decrease in <i>Slc25a39</i> mRNA in the liver and a decrease in <i>Slc25a39/40</i> mRNA and protein in the kidneys. Consequently, there was a significant decrease in mGSH levels in the kidneys of BDL mice compared with those in sham mice. LPS treatment resulted in increased <i>Slc25a40</i2022-01-01T00:00:00Z2022 Aug2025-04-04T23:46:47.636Z2025-02-19T03:32:08.676ZS-EPMC93693133595570710.3390/ijms23158573