biostudies-literatureCheng YJMinistry of Science and Technology, TaiwanCouncil of Agriculture, Taiwan2064https://www.ebi.ac.uk/biostudies/studies/S-EPMC9370353biostudies-literatureUnknown11(15)Anthracnose caused by <i>Colletotrichum</i> leads to a tremendous post-harvest mango loss. While chemical fungicides are applied to control anthracnose, natural alternatives are preferred due to food safety and environmental concerns. Pomelo extract (PE) exhibits a broad spectrum of antimicrobial activities; however, its effect against anthracnose is unknown. Here we investigated the chemical profile of PE using GC-MS and the anti-anthracnose activity of PE using in vitro and in vivo assays. We also evaluated the impact of storage temperature (0°, 5°, 10°, 20°, -20°, and -80 °C) and light conditions on the composition and antifungal activity of PE. We found that PE inhibited <i>C. gloeosporioides</i> in vitro with an IC₅₀ of 3.2 mL L^-1. Applying chitosan-based coatingPlants (Basel, Switzerland)Impact of Storage Condition on Chemical Composition and Antifungal Activity of Pomelo Extract against <i>Colletotrichum gloeosporioides</i> and Anthracnose in Post-harvest Mango.PMC9370353110AS-4.2.3-CI-C6109AS-7.3.1-CI-C9109-2313-B-002-053-MY2111AS-1.3.2-ST-aSKuan YCChu YYCheng YJChen CNLee FWOu LYWu YJfalseImpact of Storage Condition on Chemical Composition and Antifungal Activity of Pomelo Extract against <i>Colletotrichum gloeosporioides</i> and Anthracnose in Post-harvest Mango.Anthracnose caused by <i>Colletotrichum</i> leads to a tremendous post-harvest mango loss. While chemical fungicides are applied to control anthracnose, natural alternatives are preferred due to food safety and environmental concerns. Pomelo extract (PE) exhibits a broad spectrum of antimicrobial activities; however, its effect against anthracnose is unknown. Here we investigated the chemical profile of PE using GC-MS and the anti-anthracnose activity of PE using in vitro and in vivo assays. We also evaluated the impact of storage temperature (0°, 5°, 10°, 20°, -20°, and -80 °C) and light conditions on the composition and antifungal activity of PE. We found that PE inhibited <i>C. gloeosporioides</i> in vitro with an IC₅₀ of 3.2 mL L^-1. Applying chitosan-based coating2022-01-01T00:00:00Z2022 Aug2025-04-04T08:44:55.194Z2025-02-19T03:32:01.295ZS-EPMC93703533595654210.3390/plants11152064