biostudies-literatureZhao YShanghai Jiao Tong University4156966https://www.ebi.ac.uk/biostudies/studies/S-EPMC9371835biostudies-literatureUnknown2022Head and neck squamous carcinoma (HNSC) is the most prevalent malignancy of the head and neck regions. Long noncoding RNAs (lncRNAs) are vital in tumorigenesis regulation. However, the role of lncRNAs in HNSC requires further exploration. Herein, through bioinformatic assays using The Cancer Genome Atlas (TCGA) datasets, rapid amplification of cDNA ends (RACE) assays, and RNA-FISH, we revealed that a novel cytoplasmic transcript, HNSC-associated transcript 1 (HNSCAT1, previously recognized as linc01269), was downregulated in tumor samples and advanced tumor stages and was also associated with favorable outcomes in HNSC. Overexpression of HNSCAT1 triggered treatment efficacy in HNSCs both <i>in vivo</i> and <i>in vitro</i>. More importantly, through high-throughput transcriptome analysis (ROxidative medicine and cellular longevityThe Long Noncoding Transcript HNSCAT1 Activates KRT80 and Triggers Therapeutic Efficacy in Head and Neck Squamous Cell Carcinoma.PMC93718358207217781772086SSMU-ZDCX20180700shslczdzk0090181620108019Li HZhang ZHuang XZan TZhao YfalseThe Long Noncoding Transcript HNSCAT1 Activates KRT80 and Triggers Therapeutic Efficacy in Head and Neck Squamous Cell Carcinoma.Head and neck squamous carcinoma (HNSC) is the most prevalent malignancy of the head and neck regions. Long noncoding RNAs (lncRNAs) are vital in tumorigenesis regulation. However, the role of lncRNAs in HNSC requires further exploration. Herein, through bioinformatic assays using The Cancer Genome Atlas (TCGA) datasets, rapid amplification of cDNA ends (RACE) assays, and RNA-FISH, we revealed that a novel cytoplasmic transcript, HNSC-associated transcript 1 (HNSCAT1, previously recognized as linc01269), was downregulated in tumor samples and advanced tumor stages and was also associated with favorable outcomes in HNSC. Overexpression of HNSCAT1 triggered treatment efficacy in HNSCs both <i>in vivo</i> and <i>in vitro</i>. More importantly, through high-throughput transcriptome analysis (R2022-01-01T00:00:00Z20222025-04-27T03:19:58.053Z2024-12-04T07:28:46.928ZS-EPMC93718353596567910.1155/2022/4156966