{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(1)"],"submitter":["Ricci S"],"funding":["Austrian Federal Ministry for Digital and Economic Affairs","Biomin","National Foundation for Research, Technology and Development","Christian Doppler Laboratory for Innovative Gut Health Concepts of Livestock"],"pubmed_abstract":["MicroRNAs (miRNAs), as important post-transcriptional regulators, are ubiquitous in various tissues. The aim of this exploratory study was to determine the presence of miRNAs in rumen fluid, and to investigate the possibility of miRNA-mediated cross-talk within the ruminal ecosystem. Rumen fluid samples from four cannulated Holstein cows were collected during two feeding regimes (forage and high-grain diet) and DNA and RNA were extracted for amplicon and small RNA sequencing. Epithelial biopsies were simultaneously collected to investigate the co-expression of miRNAs in papillae and rumen fluid. We identified 377 miRNAs in rumen fluid and 638 in rumen papillae, of which 373 were shared. Analysis of microbiota revealed 20 genera to be differentially abundant between the two feeding regimes,"],"journal":["Scientific reports"],"pagination":["13812"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9378797"],"repository":["biostudies-literature"],"pubmed_title":["Characterization of presence and activity of microRNAs in the rumen of cattle hints at possible host-microbiota cross-talk mechanism."],"pmcid":["PMC9378797"],"pubmed_authors":["Zebeli Q","Pacifico C","Rivera-Chacon R","Reisinger N","Kreuzer-Redmer S","Sener-Aydemir A","Ricci S","Petri RM","Castillo-Lopez E"],"additional_accession":[]},"is_claimable":false,"name":"Characterization of presence and activity of microRNAs in the rumen of cattle hints at possible host-microbiota cross-talk mechanism.","description":"MicroRNAs (miRNAs), as important post-transcriptional regulators, are ubiquitous in various tissues. The aim of this exploratory study was to determine the presence of miRNAs in rumen fluid, and to investigate the possibility of miRNA-mediated cross-talk within the ruminal ecosystem. Rumen fluid samples from four cannulated Holstein cows were collected during two feeding regimes (forage and high-grain diet) and DNA and RNA were extracted for amplicon and small RNA sequencing. Epithelial biopsies were simultaneously collected to investigate the co-expression of miRNAs in papillae and rumen fluid. We identified 377 miRNAs in rumen fluid and 638 in rumen papillae, of which 373 were shared. Analysis of microbiota revealed 20 genera to be differentially abundant between the two feeding regimes,","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Aug","modification":"2025-06-01T02:33:41.871Z","creation":"2024-11-15T06:51:04.01Z"},"accession":"S-EPMC9378797","cross_references":{"pubmed":["35970850"],"doi":["10.1038/s41598-022-17445-z"]}}