biostudies-literatureAdu Mensah DEuropean and Developing Countries Clinical Trials PartnershipDeutsche ForschungsgemeinschaftGerman Federal Ministry of Education and Research (Bundesministerium für Bildung und Forschung BMBF),e0010129https://www.ebi.ac.uk/biostudies/studies/S-EPMC9380951biostudies-literatureUnknown16(8)<h4>Background</h4>Lymphatic filariasis (LF) causes chronic morbidity, which usually manifests as lymphedema or hydrocele. Mass drug administration (MDA) began in Kassena Nankana East Municipal (KNEM) and Nabdam, two hotspot districts in the Upper East Region in Ghana, in 2000 and 2005, respectively. This cross-sectional study evaluated the impact of 15 years of MDA on the control of LF as determined by circulating filarial antigen (CFA) and microfilariae assessment in the KNEM and the Nabdam districts.<h4>Methodology/principal findings</h4>A total of 7,453 participants from eight sub-districts in the two hotspot districts (KNEM: N = 4604; Nabdam: N = 2849) were recruited into the study. The overall CFA prevalence as determined by the FTS was 19.6% and 12.8% in the KNEM and Nabdam districtPLoS neglected tropical diseasesOccurrence of Lymphatic Filariasis infection after 15 years of mass drug administration in two hotspot districts in the Upper East Region of Ghana.PMC9380951TMA2018SF-245181204851PF 673/6-1Adu Mensah DBoateng JObeng PHoerauf AGyamfi PARahamani AADebrah AYOsei-Mensah JDebrah LBKlarmann-Schulz UKroidl IOpoku VSfalseOccurrence of Lymphatic Filariasis infection after 15 years of mass drug administration in two hotspot districts in the Upper East Region of Ghana.<h4>Background</h4>Lymphatic filariasis (LF) causes chronic morbidity, which usually manifests as lymphedema or hydrocele. Mass drug administration (MDA) began in Kassena Nankana East Municipal (KNEM) and Nabdam, two hotspot districts in the Upper East Region in Ghana, in 2000 and 2005, respectively. This cross-sectional study evaluated the impact of 15 years of MDA on the control of LF as determined by circulating filarial antigen (CFA) and microfilariae assessment in the KNEM and the Nabdam districts.<h4>Methodology/principal findings</h4>A total of 7,453 participants from eight sub-districts in the two hotspot districts (KNEM: N = 4604; Nabdam: N = 2849) were recruited into the study. The overall CFA prevalence as determined by the FTS was 19.6% and 12.8% in the KNEM and Nabdam district2022-01-01T00:00:00Z2022 Aug2025-04-21T22:27:51.993Z2025-04-05T18:49:01.045ZS-EPMC93809513592601210.1371/journal.pntd.0010129