{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["181(12)"],"submitter":["Voorhorst WG"],"pubmed_abstract":["The genetic organization, expression, and regulation of the celB locus of the hyperthermophilic archaeon Pyrococcus furiosus were analyzed. This locus includes the celB gene, which codes for an intracellular beta-glucosidase, and a divergently orientated gene cluster, adhA-adhB-lamA, which codes for two alcohol dehydrogenases and an extracellular beta-1,3-endoglucanase that is transcribed as a polycistronic messenger (the lamA operon). During growth of P. furiosus on either the beta-1,4-linked glucose dimer cellobiose or the beta-1,3-linked glucose polymer laminarin, the activities of both beta-glucosidase and endoglucanase were increased at least fivefold compared with levels during growth on maltose or pyruvate. Northern blot analysis revealed an enhanced transcription of both the celB g"],"journal":["Journal of bacteriology"],"pagination":["3777-83"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC93856"],"repository":["biostudies-literature"],"pubmed_title":["Transcriptional regulation in the hyperthermophilic archaeon Pyrococcus furiosus: coordinated expression of divergently oriented genes in response to beta-linked glucose polymers."],"pmcid":["PMC93856"],"pubmed_authors":["de Vos WM","Voorhorst WG","Geerling AC","Gueguen Y","Thomm M","Schut G","Dahlke I","van der Oost J"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptional regulation in the hyperthermophilic archaeon Pyrococcus furiosus: coordinated expression of divergently oriented genes in response to beta-linked glucose polymers.","description":"The genetic organization, expression, and regulation of the celB locus of the hyperthermophilic archaeon Pyrococcus furiosus were analyzed. This locus includes the celB gene, which codes for an intracellular beta-glucosidase, and a divergently orientated gene cluster, adhA-adhB-lamA, which codes for two alcohol dehydrogenases and an extracellular beta-1,3-endoglucanase that is transcribed as a polycistronic messenger (the lamA operon). During growth of P. furiosus on either the beta-1,4-linked glucose dimer cellobiose or the beta-1,3-linked glucose polymer laminarin, the activities of both beta-glucosidase and endoglucanase were increased at least fivefold compared with levels during growth on maltose or pyruvate. Northern blot analysis revealed an enhanced transcription of both the celB g","dates":{"release":"1999-01-01T00:00:00Z","publication":"1999 Jun","modification":"2025-04-04T13:22:35.727Z","creation":"2019-03-27T00:18:32Z"},"accession":"S-EPMC93856","cross_references":{"pubmed":["10368153"],"doi":["10.1128/JB.181.12.3777-3783.1999"]}}