biostudies-literatureFan LAmerican Cancer SocietyNational Institutes of HealthNIGMS NIH HHS1275-1290https://www.ebi.ac.uk/biostudies/studies/S-EPMC9385889biostudies-literatureUnknown76(5)<h4>Background and aims</h4>Hepatoblastoma (HB) is the most common pediatric liver cancer. Its predominant occurrence in very young children led us to investigate whether the neonatal liver provides a protumorigenic niche to HB development.<h4>Approach and results</h4>HB development was compared between orthotopic transplantation models established in postnatal day 5 (P5) and 60 (P60) mice (P5^Tx and P60^Tx models). Single-cell RNA-sequencing (sc-RNAseq) was performed using tumor and liver tissues from both models and the top candidate cell types and genes identified are investigated for their roles in HB cell growth, migration, and survival.<h4>Conclusions</h4>We found that various HB cell lines including HepG2 cells were consistently and considerably more tumorigenic Hepatology (Baltimore, Md.)A developmentally prometastatic niche to hepatoblastoma in neonatal liver mediated by the Cxcl1/Cxcr2 axis.PMC9385889R01 GM134382GM134382RSG‐18‐026‐01Brown AShin SEaston JTian CYu JLi LJu BKoo SCYang WYang JJLu MBondoc AZhu LFan LPan QRanganathan SfalseA developmentally prometastatic niche to hepatoblastoma in neonatal liver mediated by the Cxcl1/Cxcr2 axis.<h4>Background and aims</h4>Hepatoblastoma (HB) is the most common pediatric liver cancer. Its predominant occurrence in very young children led us to investigate whether the neonatal liver provides a protumorigenic niche to HB development.<h4>Approach and results</h4>HB development was compared between orthotopic transplantation models established in postnatal day 5 (P5) and 60 (P60) mice (P5^Tx and P60^Tx models). Single-cell RNA-sequencing (sc-RNAseq) was performed using tumor and liver tissues from both models and the top candidate cell types and genes identified are investigated for their roles in HB cell growth, migration, and survival.<h4>Conclusions</h4>We found that various HB cell lines including HepG2 cells were consistently and considerably more tumorigenic 2022-01-01T00:00:00Z2022 Nov2026-05-09T14:02:21.044Z2025-04-04T22:50:00.33ZS-EPMC93858893517979910.1002/hep.32412