biostudies-literatureCarberry CKNIEHS NIH HHS107419https://www.ebi.ac.uk/biostudies/studies/S-EPMC9389917biostudies-literatureUnknown167<h4>Introduction</h4>Wildfires are a threat to public health world-wide that are growing in intensity and prevalence. The biological mechanisms that elicit wildfire-associated toxicity remain largely unknown. The potential involvement of cross-tissue communication via extracellular vesicles (EVs) is a new mechanism that has yet to be evaluated.<h4>Methods</h4>Female CD-1 mice were exposed to smoke condensate samples collected from the following biomass burn scenarios: flaming peat; smoldering peat; flaming red oak; and smoldering red oak, representing lab-based simulations of wildfire scenarios. Lung tissue, bronchoalveolar lavage fluid (BALF) samples, peripheral blood, and heart tissues were collected 4 and 24 h post-exposure. Exosome-enriched EVs were isolated from plasma, physically chaEnvironment internationalWildfires and extracellular vesicles: Exosomal MicroRNAs as mediators of cross-tissue cardiopulmonary responses to biomass smoke.PMC9389917R21 ES031740T32 ES007018P30 ES010126P42 ES031007Hartwell HCarberry CKJaspers IIan Gilmour MRager JEPayton AReif DMSmith GJHo Kim YKoval LEfalseWildfires and extracellular vesicles: Exosomal MicroRNAs as mediators of cross-tissue cardiopulmonary responses to biomass smoke.<h4>Introduction</h4>Wildfires are a threat to public health world-wide that are growing in intensity and prevalence. The biological mechanisms that elicit wildfire-associated toxicity remain largely unknown. The potential involvement of cross-tissue communication via extracellular vesicles (EVs) is a new mechanism that has yet to be evaluated.<h4>Methods</h4>Female CD-1 mice were exposed to smoke condensate samples collected from the following biomass burn scenarios: flaming peat; smoldering peat; flaming red oak; and smoldering red oak, representing lab-based simulations of wildfire scenarios. Lung tissue, bronchoalveolar lavage fluid (BALF) samples, peripheral blood, and heart tissues were collected 4 and 24 h post-exposure. Exosome-enriched EVs were isolated from plasma, physically cha2022-01-01T00:00:00Z2022 Sep2026-05-31T16:02:38.667Z2025-02-19T01:27:42.798ZS-EPMC93899173586323910.1016/j.envint.2022.107419