{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(37)"],"submitter":["Xu T"],"pubmed_abstract":["Vitexin is an active component of many traditional chinese medicines, and is found in various plants. The low solubility of vitexin limits its pharmaceutical usage. In this study, solvent-stable β-fructosidase was used to glycosylate vitexin in organic solvents. The β-fructosidase showed high activity and stability in 30-80% (v/v) ethyl acetate with 90-99% yields of vitexin glycosides. Highly efficient synthesis of β-d-fructofuranosyl-(2→6)-vitexin (1.04 g L<sup>-1</sup>) and β-d-difructofuranosyl-(2→6)-vitexin (0.45 g L<sup>-1</sup>) was attained in 50% (v/v) ethyl acetate solvent system from 1.5 g L<sup>-1</sup> vitexin. Two novel vitexin glycosides showed higher anti-tumor activities compared to that of vitexin by employing a human breast cancer cytotoxicity assay."],"journal":["RSC advances"],"pagination":["23839-23844"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9396635"],"repository":["biostudies-literature"],"pubmed_title":["Enzymatic synthesis of vitexin glycosides and their activity."],"pmcid":["PMC9396635"],"pubmed_authors":["Du Q","Fan Z","Lu Y","Xu T","Kong Y","Lou J","Wu X"],"additional_accession":[]},"is_claimable":false,"name":"Enzymatic synthesis of vitexin glycosides and their activity.","description":"Vitexin is an active component of many traditional chinese medicines, and is found in various plants. The low solubility of vitexin limits its pharmaceutical usage. In this study, solvent-stable β-fructosidase was used to glycosylate vitexin in organic solvents. The β-fructosidase showed high activity and stability in 30-80% (v/v) ethyl acetate with 90-99% yields of vitexin glycosides. Highly efficient synthesis of β-d-fructofuranosyl-(2→6)-vitexin (1.04 g L<sup>-1</sup>) and β-d-difructofuranosyl-(2→6)-vitexin (0.45 g L<sup>-1</sup>) was attained in 50% (v/v) ethyl acetate solvent system from 1.5 g L<sup>-1</sup> vitexin. Two novel vitexin glycosides showed higher anti-tumor activities compared to that of vitexin by employing a human breast cancer cytotoxicity assay.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Aug","modification":"2025-04-27T01:09:15.454Z","creation":"2025-04-06T18:06:21.964Z"},"accession":"S-EPMC9396635","cross_references":{"pubmed":["36093238"],"doi":["10.1039/d2ra04408k"]}}