biostudies-literatureUnknown101(10)Allison JRWellcome TrustOral microbes are dispersed during dental treatment and reduction methods have been proposed, but dental unit waterline (DUWL) disinfectants have received little attention; specifically, the effect on viruses has not been studied. This study aims to 1) investigate the effect of DUWL disinfectants on viral dispersion in dental bioaerosols and 2) establish a dual-tracer system using live bacteriophage and fluorescein supported by optical particle measurement. Bacteriophage MS2 was used as a viral tracer and fluorescein as a fluorescent tracer. Validation experiments were conducted to exclude interference of one tracer with the other or of DUWL disinfectants on detection methods. Simulated "saliva" containing the tracers was infused into the mouth of a dental mannequin during 10-min dental prJournal of dental research1198-1204https://www.ebi.ac.uk/biostudies/studies/S-EPMC9397394biostudies-literatureWaterline Disinfectants Reduce Dental Bioaerosols: A Multitracer Validation.PMC9397394Allison JRNile CHolliday RDowson CDurham JJakubovics NSfalseWaterline Disinfectants Reduce Dental Bioaerosols: A Multitracer Validation.Oral microbes are dispersed during dental treatment and reduction methods have been proposed, but dental unit waterline (DUWL) disinfectants have received little attention; specifically, the effect on viruses has not been studied. This study aims to 1) investigate the effect of DUWL disinfectants on viral dispersion in dental bioaerosols and 2) establish a dual-tracer system using live bacteriophage and fluorescein supported by optical particle measurement. Bacteriophage MS2 was used as a viral tracer and fluorescein as a fluorescent tracer. Validation experiments were conducted to exclude interference of one tracer with the other or of DUWL disinfectants on detection methods. Simulated "saliva" containing the tracers was infused into the mouth of a dental mannequin during 10-min dental pr2022-01-01T00:00:00Z2022 Sep2025-04-04T21:33:48.504Z2025-04-04T21:33:48.504ZS-EPMC93973943549201610.1177/00220345221093522