{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["14(1)"],"submitter":["Qi-Xiang M"],"funding":["This study was funded by grants from the the Clinical Research Cultivating Program of Shanghai Hospital Development Center"],"pubmed_abstract":["Toll-like receptor 4 (TLR4) has been identified as a potentially promising therapeutic target in acute pancreatitis (AP). However, the role of intestinal TLR4 in AP and AP-associated gut injury remains unclear. This study aimed to explore the relationship between intestinal TLR4 and gut microbiota during AP. A mouse AP model was establish by intraperitoneal injection of L-arginine. Pancreatic injury and intestinal barrier function were evaluated in wild-type and intestinal epithelial TLR4 knockout (TLR4ΔIEC) mice. Gut microbiota was analyzed by 16S rRNA sequencing. Quadruple antibiotics were applied to induce microbiota-depleted mice. Differentially expressed genes in gut were detected by RNA sequencing. <i>L. reuteri</i> treatment was carried out in <i>vivo</i> and <i>vitro</i> study. Com"],"journal":["Gut microbes"],"pagination":["2112882"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9397436"],"repository":["biostudies-literature"],"pubmed_title":["Intestinal TLR4 deletion exacerbates acute pancreatitis through gut microbiota dysbiosis and Paneth cells deficiency."],"pmcid":["PMC9397436"],"pubmed_authors":["Nuo-Ming Y","Chun-Lan H","Bin-Qiang X","Rui-Long W","Ze-Hua H","Yang F","Qi-Xiang M","Jun-Jie F","Yue Z"],"additional_accession":[]},"is_claimable":false,"name":"Intestinal TLR4 deletion exacerbates acute pancreatitis through gut microbiota dysbiosis and Paneth cells deficiency.","description":"Toll-like receptor 4 (TLR4) has been identified as a potentially promising therapeutic target in acute pancreatitis (AP). However, the role of intestinal TLR4 in AP and AP-associated gut injury remains unclear. This study aimed to explore the relationship between intestinal TLR4 and gut microbiota during AP. A mouse AP model was establish by intraperitoneal injection of L-arginine. Pancreatic injury and intestinal barrier function were evaluated in wild-type and intestinal epithelial TLR4 knockout (TLR4ΔIEC) mice. Gut microbiota was analyzed by 16S rRNA sequencing. Quadruple antibiotics were applied to induce microbiota-depleted mice. Differentially expressed genes in gut were detected by RNA sequencing. <i>L. reuteri</i> treatment was carried out in <i>vivo</i> and <i>vitro</i> study. Com","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jan-Dec","modification":"2025-08-31T03:17:33.443Z","creation":"2025-04-06T07:50:21.892Z"},"accession":"S-EPMC9397436","cross_references":{"pubmed":["35982604"],"doi":["10.1080/19490976.2022.2112882"]}}