biostudies-literatureUnknown23(15)Liang YRibonuclease S (RNase S) is an enzyme that exhibits anticancer activity by degrading RNAs within cancer cells; however, the cellular uptake efficiency is low due to its small molecular size. Here we generated RNase S-decorated artificial viral capsids with a size of 70-170 nm by self-assembly of the β-annulus-S-peptide followed by reconstitution with S-protein at neutral pH. The RNase S-decorated artificial viral capsids are efficiently taken up by HepG2 cells and exhibit higher RNA degradation activity in cells compared with RNase S alone. Cell viability assays revealed that RNase S-decorated capsids have high anticancer activity comparable to that of standard anticancer drugs.Chembiochem : a European journal of chemical biologye202200220https://www.ebi.ac.uk/biostudies/studies/S-EPMC9400862biostudies-literatureAnticancer Activity of Reconstituted Ribonuclease S-Decorated Artificial Viral Capsid.PMC9400862Inaba HFurukawa HLiang YSakamoto KMatsuura KfalseAnticancer Activity of Reconstituted Ribonuclease S-Decorated Artificial Viral Capsid.Ribonuclease S (RNase S) is an enzyme that exhibits anticancer activity by degrading RNAs within cancer cells; however, the cellular uptake efficiency is low due to its small molecular size. Here we generated RNase S-decorated artificial viral capsids with a size of 70-170 nm by self-assembly of the β-annulus-S-peptide followed by reconstitution with S-protein at neutral pH. The RNase S-decorated artificial viral capsids are efficiently taken up by HepG2 cells and exhibit higher RNA degradation activity in cells compared with RNase S alone. Cell viability assays revealed that RNase S-decorated capsids have high anticancer activity comparable to that of standard anticancer drugs.2022-01-01T00:00:00Z2022 Aug2025-04-04T03:26:13.82Z2025-04-04T03:26:13.82ZS-EPMC94008623567620110.1002/cbic.202200220