{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Meyer LE"],"funding":["Danmarks Frie Forskningsfond"],"pagination":["e202203823"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9400879"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["61(31)"],"pubmed_abstract":["The applicability of a thermomorphic multiphasic system (TMS) composed of a hydrophobic deep eutectic solvent (DES) and an aqueous potassium phosphate buffer with a lower critical solution temperature (LCST) phase change for homogeneous biocatalysis was investigated. A lidocaine-based DES with the fatty acid oleic acid as a hydrogen-bond donor was studied. Phase diagrams were determined and presented within this study. We tested different additional components to the solvent system and observed a decrease in the cloud point of approximately 0.026 °C per concentration unit. Distribution studies revealed a clear distribution of the protein in the aqueous buffer phase (>95 %), whereas the hydrophobic substrate and educt accumulated (>95 %) in the DES-enriched layer. Finally, a reduction catal"],"journal":["Angewandte Chemie (International ed. in English)"],"pubmed_title":["A Deep Eutectic Solvent Thermomorphic Multiphasic System for Biocatalytic Applications."],"pmcid":["PMC9400879"],"funding_grant_id":["9063 00031B"],"pubmed_authors":["Meyer LE","Andersen MB","Kara S"],"additional_accession":[]},"is_claimable":false,"name":"A Deep Eutectic Solvent Thermomorphic Multiphasic System for Biocatalytic Applications.","description":"The applicability of a thermomorphic multiphasic system (TMS) composed of a hydrophobic deep eutectic solvent (DES) and an aqueous potassium phosphate buffer with a lower critical solution temperature (LCST) phase change for homogeneous biocatalysis was investigated. A lidocaine-based DES with the fatty acid oleic acid as a hydrogen-bond donor was studied. Phase diagrams were determined and presented within this study. We tested different additional components to the solvent system and observed a decrease in the cloud point of approximately 0.026 °C per concentration unit. Distribution studies revealed a clear distribution of the protein in the aqueous buffer phase (>95 %), whereas the hydrophobic substrate and educt accumulated (>95 %) in the DES-enriched layer. Finally, a reduction catal","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Aug","modification":"2025-04-04T21:52:15.806Z","creation":"2025-04-04T21:52:15.806Z"},"accession":"S-EPMC9400879","cross_references":{"pubmed":["35587655"],"doi":["10.1002/anie.202203823"]}}